链式电喷射电离化质谱仪用于超低体积样本分析
Yu Wang1, Lu Chen1, Xia Huang1
1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China; University of Chinese Academy of Sciences, Beijing, 101408, China.
Talanta
|June 14, 2024
概括
链式电喷射电离 (链式ESI) 能够对微量样本进行高度敏感的质谱分析. 这种新方法使用极低的样本量,提供了广泛的代谢物数据,并保留了分析物的完整性,以获得准确的生物学见解.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 质谱测量质量谱测量
背景情况:
- 传统的质谱法需要大量的样本,限制了稀缺的生物材料的分析.
- 电子喷射电离 (ESI) 是一种常见的技术,但可能会受到电解氧反应的影响,改变分析物的状态.
研究的目的:
- 开发一种新的电离技术,链式喷射电离 (链式ESI),用于高效的微量样本分析.
- 为了使敏感的质谱分析能够在最小的样本消耗和原生分析物状态的保存下进行分析.
主要方法:
- 链式电喷离电离 (链式ESI) 作为主要离子源的开发.
- 使用链式ESI以每分钟 (pLs/min) 消耗率诱导二次电喷气电离.
- 来自单个生物样本的代谢物分析,例如Broussonetia papyrifera三合体和A549癌细胞.
主要成果:
- 链式ESI显示了非常低的样本消耗率 (pLs/min).
- 实质性的双重质谱 (MS2) 数据是从小体积样本中生成的,以促进代谢物注释.
- 链式ESI有效地防止了电解氧反应,确保了分析物的原始状态.
- 从单个B. papyrifera三体中检测到1426种代谢物,从单个A549癌细胞中检测到617种代谢物.
结论:
- 链-ESI提供生物样本的直接,快速分析,其体积极低,代谢物覆盖率高.
- 这种技术可以从有限的样本数量中测量关键的生物信息.
- 链式ESI代表了在微量样本上的基于质谱的代谢学方面的重大进步.
相关概念视频
Electrospray Ionization (ESI) Mass Spectrometry
780
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
780
Chemical Ionization (CI) Mass Spectrometry
727
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
727
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
703
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
703
Mass Spectrometry: Complex Analysis
743
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
743
Capillary Electrophoresis: Applications
375
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
375
Capillary Electrophoresis: Instrumentation
215
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
215


