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在清细胞细胞癌的类转变中,表皮细胞-介质细胞转换相关的标志物
Tanja Čugura1, Emanuela Boštjančič1, Sara Uhan1
1Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Experimental and molecular pathology
|June 14, 2024
概括
表皮-介质细胞过渡 (EMT) 是癌症的关键. 这项研究发现类细胞癌 (sRCC) 显示出分子差异,表明部分EMT,而不是完全EMT,驱动其进展.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 表皮-介质细胞过渡 (EMT) 对于癌症的发展和扩散至关重要.
- 部分EMT (pEMT) 与瘤迁移有关.
- 类脏细胞癌 (sRCC) 是一种侵略性的亚型,不清楚EMT的参与.
研究的目的:
- 调查 (p) EMT在细胞癌 (RCC) 进展到sRCC中的作用.
- 为了分析EMT标志物表达在清晰细胞RCC (ccRCC) 和sRCC.
主要方法:
- 定量PCR (qPCR) 用于评估EMT标记物 (miR-200家族,miR-205,SNAI1/2,TWIST1/2,ZEB1/2,CDH1/2,VIM). 通过定量PCR检测,可以测量EMT标记物 (miR-200家族,miR-205,SNAI1/2,TWIST1/2,ZEB1/2,CDH1/2,VIM).
- 对于E-cadherin,N-cadherin,vimentin和ZEB2的免疫组织化学.
- 在ccRCC,sRCC组件 (sRCC-Ca,sRCC-Sa) 和非新形成组织之间对标记表达的比较.
主要成果:
- miR-200c在sRCC-Ca和ccRCC下调;miR-200a在sRCC-Sa和ccRCC下调. 在sRCC-Ca和ccRCC下调. 在sRCC-Sa和ccRCC下调.
- 与所有其他组相比,在sRCC-Sa中CDH1 (E-cadherin) 的调节下降.
- 在ccRCC和sRCC中降低ZEB2的调节,与非新发性脏相比.
- 在CDH1和miR-200a/b/c表达之间存在正相关性.
结论:
- 完全的EMT很可能不在sRCC中.
- 在ccRCC,sRCC-Ca和sRCC-Sa中,不同的分子概况表明了pEMT的中间状态.
- 了解这些状态可以澄清sRCC进展机制.
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