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在Rhodospirillum rubrum中通过合成气驱动的光异质变性适应性进化促进的生产

Natalia Hernández-Herreros¹, Alberto Rodríguez¹, Beatriz Galán²

¹Microbial & Plant Biotechnology Department, Polymer Biotechnology Group, Biological Research Centre Margarita Salas, Spanish National Research Council (CIB-CSIC), Madrid, Spain; Interdisciplinary Platform for Sustainable Plastics towards a Circular Economy-Spanish National Research Council (SusPlast-CSIC), Madrid, Spain.

Bioresource technology

|June 14, 2024

概括

这项研究使用适应性实验室进化增强了Rhodospirillum rubrum中的 (H2) 和多基酸盐 (PHA) 生产. 进化的菌株显示,从合成气中改善了聚-3-基酸盐 (PHB) 和H2的合成.

科学领域:

微生物学微生物学
生物技术是生物技术.
代谢工程是代谢工程.

背景情况:

Rhodospirillum rubrum是一种光合作用细菌,为废物利用提供了代谢的多功能性.
由R. rubrum发酵的合成气是生产有价值化合物的潜在途径.
优化R. rubrum以提高 (H2) 和多甲基酸盐 (PHA) 的生产对于生物技术应用至关重要.

研究的目的:

研究R. rubrum在光异质变条件下从合成气中产生H2和PHA的过程.
通过适应性实验室进化 (ALE) 改善H2和PHA产量.
分析进化菌株的基因组和蛋白质组变化.

主要方法:

采用一种适应性实验室进化 (ALE) 策略,超过200代.
在光异质变条件下,在合成气体上种植R. rubrum.
进化菌株 (PA变种) 的基因组和蛋白质组分析.

主要成果:

两个进化的R. rubrum菌株表现出减少滞后期和增强的聚-3-基酸盐 (PHB) 和H2的产生.
基因组分析揭示了四个基因的单点突变,包括PpsR调节器.
蛋白质组数据表明,通过CO-脱酶复合体加强了CO的氧化,通过光合作用系统改善了能量获取.

结论:

ALE有效地提高了R. rubrum中的H2和PHB产量.
关键调节和代谢基因的突变有助于提高性能.
进化的R. rubrum菌株显示出可持续的合成气生物转换的巨大潜力.

关键词:

对于CODH复合物来说,它是一种复合物.基因组学就是基因组学.光合作用光合作用蛋白质组学是指蛋白质组学.紫色的非硫细菌紫色的非硫细菌

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