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Updated: Jun 23, 2025

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Bovine Ovarian Cortex Tissue Culture
Published on: January 14, 2021
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凯托可纳阻断孕激素的产生,而不会影响积卵细胞复合体成熟的其他参数
K Asimaki1, P Vazakidou2, H T A van Tol3
1Division of Farm Animal Health, Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands; Amsterdam Institute for Life and Environment, Section Environment and Health, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands.
Reproductive toxicology (Elmsford, N.Y.)
|June 14, 2024
概括
凯托可纳 (KTZ) 可以阻断牛卵细胞中的类固醇激素合成. 然而,KTZ并没有显著影响卵细胞成熟或早期胚胎发育,这表明该模型的生殖毒性较低.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 内分泌学 在内分泌学.
背景情况:
- 凯托可纳 (KTZ) 是一种已知抑制类固醇生成的杀菌剂.
- 对女性生殖健康的潜在影响需要调查.
- 牛累积卵细胞复合体 (COCs) 作为生殖毒性研究的有价值模型.
研究的目的:
- 评估基托可纳 (KTZ) 对牛累积卵细胞复合体 (COCs) 体外成熟的影响.
- 评估KTZ对类固醇激素产生,卵细胞成熟,亡和早期胚胎发育的影响.
- 为了确定KTZ在牛体外成熟模型中的生殖毒性.
主要方法:
- 牛的COC在体外成熟,KTZ度各不相同 (10-6M到10-8M).
- 测量了类固醇生成 (孕,孕) 的情况.
- 评估了卵细胞核成熟,累积扩张,亡和亡.
- 受精卵子经过胚胎培养8天,有或没有KTZ暴露.
主要成果:
- 凯托可纳在10-6M时显著抑制了孕激素和孕激素的合成.
- 较低的KTZ度 (10-7M,10-8M) 并没有影响类固醇生成.
- 卵细胞成熟,累积扩张,亡和亡不受KTZ的影响.
- 早期胚胎发育在很大程度上不受影响,尽管在10-8 M KTZ时注意到胚胎细胞形成的减少.
- 当KTZ仅在培养阶段存在时,胚胎发育不会受到不利影响.
结论:
- 凯托可纳在较高度下有效地阻断牛的COC中的类固醇生成.
- 在牛群中,KTZ不会显著影响体外卵子成熟或早期胚胎发育.
- 该研究表明,在牛模型中,尽管抑制了类固醇生成,但基托可纳的生殖毒性有限.
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