核桃蛋白分离-epigallocatechin gallate纳米颗粒:一种功能性载体,增强了利科的稳定性和抗氧化活性
Yuzhen Wang1, Jiao Lv1, Chang Li1
1National Key Laboratory for Efficient Production of Forest Resources, Beijing Key Laboratory of Forestry Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Food research international (Ottawa, Ont.)
|June 14, 2024
概括
核桃蛋白质分离-epigallocatechin gallate纳米颗粒有效地封装了利科,增强了其稳定性和抗氧化活性. 这种新型的输送系统显示了改善食品工业中生物活性化合物保存的前景.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 营养学药物开发
背景情况:
- 像莱科这样的生物活性化合物容易降解,限制了它们的应用.
- 开发稳定的输送系统对于保持营养药的有效性至关重要.
- 核桃蛋白分离物 (WPI) 和表甲基酸盐 (EGCG) 可以形成结合物,用于创建食品级系统.
研究的目的:
- 开发WPI-EGCG纳米颗粒 (WENPs) 用于封装柳科 (LYC).
- 为了评估封装式利科的稳定性,释放动力学和抗氧化特性.
- 研究WENPs/LYC.抗氧化作用背后的分子机制.
主要方法:
- 超声波辅助方法用于WPI-EGCG纳米粒子合成和烯封装.
- 韦恩普/LYC的物理化学特征,包括大小和形态.
- 使用HepG2细胞进行体外抗氧化活性测定和氧化应激标志物的评估.
- 转录组分析和qRT-PCR以阐明与抗氧化途径相关的基因表达.
主要成果:
- 成功形成了具有受控尺寸的WENPs/LYC (200-300 nm长,直径14.81-30.05 nm).
- 增强了对热,紫外线和储存条件的利科稳定性,释放率降低.
- 与自由烯 (EC50 = 31.54 μg/mL) 相比,WENPs/LYC的协同作用和优越的抗氧化活性 (EC50 = 23.98 μg/mL).
- 恢复了HepG2细胞中的抗氧化酶活性 (SOD,CAT,GSH-Px) 并抑制了MDA的形成.
- 转录组分析显示了谷氨代谢和过氧体路径的丰富.
结论:
- WPI-EGCG纳米颗粒提供了高的有效输送系统,增强了其稳定性和生物活性.
- WENPs/LYC显示出显著的抗氧化潜力,并调节细胞抗氧化剂防御机制.
- 这项技术为食品工业提供了有前途的应用,用于保存和输送生物活性化合物.
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