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一种高度敏感的光生物传感器,用于基于聚乙烯脂质体的亚毒素B₁检测,与外核酶III辅助循环放大相结合

Chunxu Tao¹, Junyan Wang¹, Ying Zhu¹

¹School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.

Mikrochimica acta

|June 14, 2024

概括

一种新型的光生物传感器有效地检测到使用聚乙烯脂质体和外核酶III放大功能的阿弗拉托克辛B1. 这种方法在各种食品样本中提供了灵敏而准确的阿弗拉托克辛B1检测.

科学领域:

生物技术是生物技术.
分析化学分析化学
生物感应是一种生物感应.

背景情况:

亚毒素B1 (AFB1) 对健康构成重大风险.
准确而敏感的AFB1检测方法对于食品安全至关重要.

研究的目的:

开发一种新的光生物传感器,用于AFB1测定.
为了提高灵敏度,利用聚乙烯 (PDA) 脂质体和外核酶III (EXO III) 辅助的循环放大.

主要方法:

开发了一种生物传感器,集成了AFB1吸收体,辅助DNA (cDNA) 和发针DNA (H).
使用EXO III进行cDNA的循环放大,生成捕获DNA.
利用捕获DNA触发探针修饰的PDA脂质体的聚合,从而产生光信号.

主要成果:

实现了AFB1的0.18 ng/mL的检测极限,在1-100 ng/mL的线性范围内.
在真实食品样本中显示出高回收率 (92.81-106.45%) 和较低的相对标准偏差 (1.73-4.26%).
证实了方法的稳定性,准确性和实际应用的特异性.

结论:

开发的光生物传感器为AFB1检测提供了灵敏和可靠的方法.
PDA脂质体和EXO III放大组合显示出食品安全分析的巨大潜力.
该方法适用于对玉米,大米,花生和小麦等各种食品矩阵的AFB1分析.

关键词:

非洲毒素B1是什么?亚普特美尔 (Aptamer) 是一种药物.异核酶III可以产生异核酶.光是一种光效应.脂质细胞体中的脂肪体.

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