PsSOC1参与了吉伯雷林通路,在树的内臭性释放过程中触发细胞增殖和芽
Yuxi Zhang1,2, Demei Niu1,2, Yanchao Yuan1,2
1College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
The New phytologist
|June 15, 2024
概括
树中的吉伯雷林 (GA) 信号涉及PsRGL1和PsSOC1蛋白质. 这项研究揭示了PsSOC1如何与PsRGL1相互作用,通过调节下游基因来促进芽休眠释放.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 激素信号传递的激素.
背景情况:
- 树 (Paeonia suffruticosa) 呈现了芽内臭,这是由吉伯雷林 (GA) 途径调节的关键发育过程.
- 德拉蛋白PsRGL1作为芽休眠释放的负调节剂,但精确的GA信号机制尚不清楚.
研究的目的:
- 阐明GA信号释放的分子机制释放了树的芽休眠状态.
- 为了确定参与GA介导休眠释放的关键蛋白质和途径.
主要方法:
- 酵母两杂交查以确定蛋白质相互作用.
- 下拉和光酶补充成像测试以确认相互作用.
- 转基因植物试验 (树和杂交) 来评估基因功能.
- 转录组分析,促进体检测 (酵母单杂交,电泳性移动性转移检测,ChIP-qPCR,双光酶检测) 以确定和验证下游目标.
- 基因分析以验证下游目标的作用.
主要成果:
- PsSOC1通过其MADS域与PsRGL1相互作用.
- PsSOC1促进了转基因树和杂交的芽休眠释放.
- PsCYCD3.3和PsEBB3被确定为PsSOC1的直接下游目标,与它们的CARG动机结合.
- PsRGL1-PsSOC1相互作用抑制了PsSOC1的DNA结合活性.
- PsCYCD3.3通过启动细胞增殖来促进芽休眠释放.
结论:
- 一个新的GA信号通路,GA-PsRGL1-PsSOC1-PsCYCDs,在树芽休眠释放中得到阐明.
- 这一途径扩大了对GA在调节植物发育过程中的作用的理解.
- PsSOC1在GA信号传递中起着关键的桥梁作用,将DELLA蛋白调节与下游基因激活联系起来,以释放休眠状态.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Primary and Secondary Growth in Roots and Shoots
57.1K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
57.1K
Meristems and Plant Growth
45.8K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
45.8K
Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
Cell Adhesion in Plants
2.7K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.7K
Morphogenesis
28.0K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.0K


