使用尼古提亚烟草 (Nicotiana tabacum) 转基因细胞植物生产生物活性的人类基本纤维细胞生长因子 (hFGFb)
Carolina Müller1, Nicolás Budnik2, Federico Gabriel Mirkin1
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres" (INGEBI-CONICET), Vuelta de Obligado 2490, Ciudad Autónoma Buenos Aires, C1428ADN, Argentina.
Planta
|June 15, 2024
概括
研究人员创造了基因改造的烟草植物,在质体中产生人类基本纤维细胞生长因子 (hFGFb). 纯化的蛋白质具有生物活性,显示了在植物中具有成本效益的重组蛋白质生产的潜力.
科学领域:
- 植物生物技术 植物生物技术
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 植物提供了一个具有成本效益的平台来生产高价值的重组蛋白质.
- 塑基因组转换使这些蛋白质能够在高水平上积累.
- 重组生长因子对于细胞培养和再生医学至关重要.
研究的目的:
- 在烟草的叶绿体中产生生物活性复合人体基本纤维细胞生长因子 (hFGFb).
- 评估植物生物反应器在hFGFb生产中的潜力.
主要方法:
- 产生表达hFGFb的跨细胞体烟草线在质细胞层中.
- 从烟草叶片中净化复合的hFGFb.
- 使用HEK293T细胞增殖试验评估rhFGFb的生物活性.
主要成果:
- 成功生成了两个独立的,同等质体的跨质体烟草生产线.
- 在这些线的叶子中确认了rhFGFb的积累.
- 纯化rhFGFb通过刺激HEK293T细胞增殖,显示出生物活性.
结论:
- 塑基因组转换是产生功能性rhFGFb的可行策略.
- 转质细胞烟草作为一种有效的植物生物反应器,用于重组生长因子的生产.
- 这项技术对具有成本效益的治疗蛋白质制造具有前景.
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