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玉米辅酶反应因子ZmARF1在转基因阿拉比多普西斯中赋予了多种非生物压力抵抗力

Ling Liu^1,2, Ying Gong^2,3, Baba Salifu Yahaya^2,3

¹Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, 644000, Sichuan, China.

Plant molecular biology

|June 15, 2024

概括

过度表达ZmARF1增强了植物对多种非生物应激的耐受性,如低,干旱和盐度. 这改善了根生长和与压力相关的基因表达,有助于作物存活.

科学领域:

植物科学植物科学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

无生物应激显著影响植物的发育和生存.
氧化应激是长期暴露于环境压力因素的主要后果.
开发具有增强耐压力的作物对于全球粮食安全至关重要.

研究的目的:

研究ZmARF1在赋予植物对低Pi,干旱和盐度压力的耐受性方面的作用.
阐明ZmARF1介导的压力耐受性背后的分子机制.
评估ZmARF1在提高作物弹性方面的潜力.

主要方法:

在玉米和Arabidopsis thaliana中过度表达ZmARF1.
在低Pi条件下对根特征的表型分析.
在干旱和盐分压力下测量生理参数 (叶绿素含量,光,水损失,离子泄漏).
转录组分析和基因表达分析 (PHT1,2,PHO2,PHR1,POX).
染色体免疫沉测定以确认ZmARF1与POX促进体结合.

主要成果:

在低Pi条件下,ZmARF1过度表达显著改善了根的表型特征 (长度,尖端,面积,体积).
转基因植物显示Pi含量增加和Pi载体 (PHT1,2) 和PSI基因 (PHO2,PHR1) 的升调.
过度表达ZmARF1的阿拉比多普西斯表现出对干旱和盐酸压力的耐受性提高,由更高的叶绿素含量,更好的叶绿素光,减少水损失和较低的离子泄漏表明.
转录组分析显示,在压力下,过氧化酶基因 (POX) 的上调.
证实ZmARF1可以结合POX促进体中的辅酶反应元件 (AuxRE),增强其转录.

结论:

ZmARF1在调解对低Pi,干旱和盐度压力的耐受性方面发挥着至关重要的作用.
在低Pi条件下,ZmARF1增强了Pi的吸收和利用.
通过调节过氧化酶基因表达,ZmARF1赋予了氧化应激耐受性.
ZmARF1显示出开发具有改善多重压力耐受性的作物的巨大潜力.

关键词:

在 ZmARF1F1 中.干旱造成的压力是干旱.低 Pi 应激压力过氧化酶基因是什么盐的压力是盐的压力.

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