相关实验视频

Updated: Jun 23, 2025

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

在体细胞核转移胚胎中,LEUTX调节了猪胚胎基因组激活

Kai Zhou¹, Tingting Wang¹, Jingjing Zhang¹

¹Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China.

|June 15, 2024

概括

配对的同源体转录因子 (TFs) 调节胚胎基因组激活 (EGA). 发现LEUTX是一种类似PRD的TF,对猪EGA至关重要,在过度表达时改善胚胎发育和克隆效率.

科学领域:

发展生物学发展生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

配对类 (PRD类) 家庭盒转录因子 (TFs) 越来越多地被认为是它们在胚胎基因组激活 (EGA) 中的作用.
动物已经失去了大多数PRD类基因,因此需要对其他哺乳动物模型进行研究.
类似PRD的TF在人类,子和猪胚胎中在EGA期间呈现短暂的表达,但在克隆胚胎中表达不足.

研究的目的:

为了研究PRD类TFs在非动物哺乳动物的EGA中的作用,使用猪作为模型.
确定涉及猪EGA的特定PRD类TF及其监管机制.
评估LEUTX在克隆猪中拯救EGA衰竭和改善早期胚胎发育的潜力.

主要方法:

在受精和克隆的猪胚胎中对PRD类TF表达的比较分析.
基因组分析,以确定关键的PRD类TFs监管EGA.
在克隆的猪胚胎中过度表达LEUTX,以评估其对EGA和发育的影响.
染色体分析以确定LEUTX的作用机制,包括招募乙转移酶.

主要成果:

包括LEUTX在内的PRD类TF在猪的EGA过程中暂时表达.
LEUTX被确定为猪EGA的关键激活剂.
莱特克斯过度表达成功恢复了EGA,增强了植入前发育,并提高了猪胚胎的克隆效率.
通过打开基因组区域,并通过p300和KAT2A招募促进基因组乙化,LEUTX促进了EGA.

结论:

莱特克斯是猪胚胎基因组激活 (EGA) 的关键调节剂.
LEUTX的机制涉及表观遗传修饰,特别是基因组乙化,以激活与EGA相关的基因.
了解LEUTX的作用可以了解早期胚胎发育和猪和潜在的其他非动物哺乳动物的克隆效率.

关键词:

科普:分子生物学分子生物学科普:干细胞研究

更多相关视频

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

相关概念视频

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...

Somatic to iPS Cell Reprogramming

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...

Introduction to Nuclear Reprogramming

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...