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苏埃达海上多糖类减弱RAW264.7细胞的LPS诱导的炎症和抗氧化活性

Weerawan Rod-In¹, Sangguan You², Woo Jung Park³

¹Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok 65000, Thailand.

International immunopharmacology

|June 15, 2024

概括

研究了Suaeda海上多糖 (SMPs) 的抗炎和抗氧化特性. 纯化的分量SMP-H3通过减少氧化和炎症性细胞因子,显示出显著的抗炎作用,表明其作为功能性食品成分的潜力.

科学领域:

海洋植物学海洋植物学
生物化学生物化学
免疫学免疫学免疫学

背景情况:

来自海洋植物的多糖因其多样化的生物活动而得到认可.
苏埃达海上多糖 (SMP) 具有潜在的治疗应用,需要进一步研究.
了解SMPs的抗炎和抗氧化特性可以导致新的功能性食品和药物开发.

研究的目的:

从Suaeda海上提取和净化多糖.
评估这些多糖的抗炎和抗氧化活性.
阐明潜在的治疗应用的潜在作用机制.

主要方法:

从Suaeda maritima中提取和净化多糖,产生SMP-C,SMP-F1和SMP-F2.
SMP-F1的水解产生SMP-H1,SMP-H2和SMP-H3的部分.
试验室试验评估RAW264.7巨细胞的抗炎作用,包括氧化 (NO) 生产,炎症调解剂和细胞因子水平以及细胞表面标记物表达.
对信号通路 (NF-κB和MAPK) 和抗氧化活性 (DPPH,超氧化物,ABTS激素清理) 的分析.

主要成果:

SMP-F1对氧化生产表现出显著的抑制作用.
与SMP-H1和SMP-H2相比,SMP-H3的水解分离显示出更好的抗炎性质.
SMP-H3显著降低了NO,iNOS,COX-2,TNF-α,IL-1β和IL-6,同时增加了IL-10,CD11b和CD40的表达.
SMP-H3调节了NF-κB和MAPK通路,并表现出强大的抗氧化活性.

结论:

来自Suaeda maritima的水解多糖体SMP-H3具有显著的抗炎和抗氧化特性.
SMP-H3的抗炎作用通过通过NF-κB和MAPK通路抑制关键的炎症媒介和细胞因子来介导.
作为功能性食品和替代治疗剂的有价值成分,SMP-H3具有前景.

关键词:

这是一种抗炎症药物.巨细胞是一个巨细胞.多糖类多糖化物.苏埃达海事公司 (Suaeda maritima)

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