在温带气候地区处理市政废水的高速无氧反应堆中,水解酶活性
Eleonora Paissoni1, Bruce Jefferson1, Ana Soares1
1Cranfield Water Science Institute, Cranfield University, Cranfield MK43 0AL, United Kingdom.
Bioresource technology
|June 15, 2024
概括
在寒冷的气候下,废水处理水解是缓慢的. 这项研究发现,厌氧膜生物反应器 (AnMBR) 中的酶在低温下保持高基质亲和力,提高效率.
科学领域:
- 环境科学 环境科学
- 生物技术是生物技术.
- 生物化学 生化学
背景情况:
- 颗粒物水解限制了温带气候下的无氧废水处理效率.
- 理论上说,低温可以减少酶基质相互作用,但这并未得到充分理解.
研究的目的:
- 研究低温对无氧处理系统中水解酶活性和动力学的影响.
- 为了比较上游无氧污泥毯 (UASB) 反应器和无氧膜生物反应器 (AnMBRs) 之间的酶动力学.
主要方法:
- 在试点规模的UASB反应器和AnMBR中评估了β-葡萄糖酶,蛋白酶和脂酶活动.
- 在操作温度 (15-20°C) 和最佳温度 (37°C) 下使用迈凯利斯-门分析确定酶动力学.
主要成果:
- 酶特异性活性在37°C达到峰值,表明缺乏心理爱好酶的主导地位.
- 迈凯利斯-门常数 (Km) 在15-20°C时显示出高酶基质亲和力,特别是在AnMBR中.
- 膜超过通过增强酶迈凯利斯-门常数,对水解产生了积极的影响.
结论:
- 水解酶亲和力适应工作温度,AnMBRs在较低的温度下表现出更大的亲和力.
- 种子污泥的适应有助于在不同类型的反应堆中保持一致的水溶性酶性亲和力.
- 膜过通过改善酶性水解动力学来提高无氧消化效率.
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