一种基于酵母的新型生物传感器,用于快速确定脱氧尼瓦伦
Han Yang1, Lianqun Du2, Lu Geng3
1Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China; School of Tea and Coffee, Pu'er University, Yunnan, 665000, PR China; School of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin, 300384, PR China.
Analytica chimica acta
|June 15, 2024
概括
一个新的酵母表面显示生物传感器有效地检测食品中的Deoxynivalenol (DON) 菌毒素. 这种灵敏而快速的方法为食品安全测试和污染物查提供了一个用户友好的方法.
科学领域:
- 免疫技术是一种免疫技术.
- 生物传感器开发开发
- 食品安全分析 食品安全分析
背景情况:
- 菌毒素,如Deoxynivalenol (DON),对人类健康构成重大风险,并在食品商品中普遍存在.
- 高特异性抗体和敏感检测方法的开发对于准确的真菌毒素分析在食品安全方面至关重要.
研究的目的:
- 通过酵母表面显示 (YSD) 提出一种用于快速选高特异抗原结合片段 (Fab) 抗体的新策略.
- 开发基于YSD的生物传感器,用于敏感和特定检测小分子化合物,以DON为例.
主要方法:
- 构建一个酵母表面显示器的构建 脱氧尼瓦enol (DON) -Fab库.
- 使用磁激活细胞分类 (MACS) 和光激活细胞分类 (FACS) 对DON选择性酵母进行选.
- 开发一个单化学发光生物传感器,利用选定的Fab@YSD C4#进行DON检测.
主要成果:
- 通过使用MACS/FACS.成功选了四种DON选择性酵母克隆.
- 开发了一种Fab@YSD化学发光生物传感器,其检测极限低 (LOD = 0.166 pg/mL),线性范围广 (0.001-132.111 ng/mL).
- 在40分钟内在各种食品样本 (小麦,玉米,面粉,玉米粉) 中检测DON的高特异性和恢复率.
结论:
- Fab@YSD化学发光生物传感器为敏感的DON检测提供了一种廉价,可复制和易于使用的方法.
- 这种基于YSD的生物传感器技术有可能快速选食品中的其他小分子污染物.
- 该研究推进了用于检测真菌毒素的免疫技术,提高了食品安全监督.
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