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皮拉德对斑马鱼下丘脑-垂体-甲状腺 (HPT) 轴的影响

Le Qian¹, Yikai Zhang¹, Xuheng Gao¹

¹College of Horticulture and plant protection, Henan University of Science and Technology, Luoyang, Henan Province, People's Republic of China.

Pesticide biochemistry and physiology

|June 15, 2024

概括

斑马鱼幼虫暴露于皮拉德农药会破坏甲状腺功能,导致发育异常和生长抑制. 这种杀虫剂干扰下丘脑-垂体-甲状腺轴和甲状腺激素受体β.

科学领域:

环境毒理学环境毒理学
内分泌学在内分泌学.
发展生物学发展生物学

背景情况:

暴露于杀虫剂可以破坏水生生物中的内分泌系统.
甲状腺激素对于脊椎动物的发育至关重要.

研究的目的:

为了研究皮拉德对斑马鱼甲状腺功能的影响.
为了阐明斑马鱼在早期发育过程中皮拉德的毒性机制.

主要方法:

斑马鱼幼虫在8天内暴露在皮拉德的次致命剂量 (0.3-1.2毫克/升) 中.
进行了下丘脑-垂体-甲状腺 (HPT) 轴组件的基因表达分析.
分析了甲状腺激素水平 (T4,T3) 和分子对接.

主要成果:

皮拉德暴露导致游泳膀尺寸,体重和发育异常的减少.
观察到crh,tg,ttr,ugt1ab的上调和trβ表达的下调.
标志着T4和T3水平的升高以及pentiopyrad对甲状腺激素受体β结合的潜在破坏.

结论:

亚致杀伤性皮拉德剂量会对斑马鱼的HPT轴产生不利影响.
皮拉德诱导斑马鱼幼虫的异常发育和生长抑制.
皮拉德通过甲状腺干扰对水生脊椎动物发育构成风险.

关键词:

在HPT轴上分子对接是分子对接.潘提奥皮拉德 (Penthiopyrad) 是一种可怕的物质.游泳膀游泳膀游泳膀斑马鱼是一种斑马鱼.

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