塔尔基因家族:在Cucumis sativus L的各种外部激素和水浸压力下进行鉴定,进化和表达分析
Sheraz Ahmad1, Khushboo Khan2, Ibrahim A Saleh3
1College of Plant Protection, Yangzhou University, 48 Wenhui East Road, Yangzhou, Jiangsu, 225009, PR China. dh20010@yzu.edu.cn.
BMC plant biology
|June 15, 2024
概括
黄瓜中的三种氨基酸环延伸 (TALE) 基因家族在植物生长和应激反应中起着至关重要的作用. 这项研究确定了21个CsTALE基因,揭示了它们的进化关系和功能,特别是在水浸压力下.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 转录法规 转录法规
背景情况:
- 三种氨基酸环延伸 (TALE) 基因是植物生长,发育和环境应激反应至关重要的家庭盒基因.
- 塔尔基因家族调节了植物中关键的生理过程.
研究的目的:
- 确定和描述黄瓜 (Cucumis sativus L.) 中的TALE基因家族.
- 研究 CsTALE 基因的进化关系,功能角色和表达模式.
- 探索 CsTALE 基因在应对水浸压力时的参与.
主要方法:
- 在整个基因组中识别CSTALE基因.
- 用于结构和功能表征的生物信息分析.
- 遗传学分析以确定进化关系.
- 对cis作用元素和蛋白质与蛋白质相互作用的分析.
- 组织特异性基因表达分析.
- 在水浸压力下进行定量表达分析.
主要成果:
- 21个CsTALE基因被确定并分为七个子类.
- 作用元素表明它们在荷尔蒙和与压力相关的途径中的作用.
- CsTALE 蛋白与其他关键转录因子相互作用.
- CsTALE15和CsTALE16分别是CsmiR319和CsmiR167a-3p的目标.
- CsTALE 基因在不同发育阶段表达.
- CsTALE17在特定的水浸压力条件下显示出显著的调节.
结论:
- 这项研究阐明了黄瓜TALE基因家族的演变和功能.
- 这些发现提供了关于 CsTALE 基因在水浸压应激反应中的作用的见解.
- 这项研究作为未来研究改善作物抗水浸能力的基础.
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