声波喷射质谱法使得超快速的蛋白质生物标记物定量化成为可能
Bart Van Puyvelde1,2, Christie L Hunter3, Maxim Zhgamadze2
1ProGenTomics, Laboratory of Pharmaceutical Biotechnology, Ghent University, 9000, Ghent, Belgium.
Nature communications
|June 15, 2024
概括
声波喷射质谱法 (AEMS) 能够超快速量化疾病生物标志物的蛋白质. 这种新分析方法显著提高了大规模生物分析查的吞吐量,有助于快速诊断.
科学领域:
- 生物化学 生化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 随着COVID-19的流行,人们发现了对高通量生物分析方法的关键需求.
- 精确的蛋白质量化对于了解健康和疾病状态至关重要.
- 像LC-MS这样的现有方法缺乏大规模查所需的速度.
研究的目的:
- 为超快速生物标志物查提供定量声喷射质谱 (AEMS) 试验.
- 为了证明AEMS的应用与免疫捕获用于血和鼻拭子中的蛋白质定量.
- 评估AEMS试验的速度,可重复性和稳定性.
主要方法:
- 开发了一种定量AEMS试验,利用免疫捕获进行分析剂丰富.
- 在267个人体血样本中应用了测量方法来量化10个急性阶段响应 (APR) 蛋白标记物.
- 在145个鼻拭片中应用了测量方法来定量SARS-CoV-2核酸 (NCAP) .
主要成果:
- 在4.8小时内量化了血样本中的10个APR蛋白,其%CV从4.2%到10.5%不等.
- 在短短10分钟内从病毒擦拭物中量化SARS-CoV-2 NCAP.
- 与传统的LC-MS相比,速度提高了15倍,在10,000次测量中表现稳定.
结论:
- 开发的AEMS试验提供了超高吞吐量和可重复的定量生物标志物查.
- 这种方法适用于分析非常大的患者队列,增强诊断能力.
- 与类免疫捕获相结合的AEMS代表了生物分析实验室能力的重大进步.
更多相关视频
14:51Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Published on: November 13, 2021
5.2K
07:49Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
80.7K
相关概念视频
Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
MALDI-TOF Mass Spectrometry
4.8K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
4.8K
Electrospray Ionization (ESI) Mass Spectrometry
780
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
780
Mass Spectrometry: Complex Analysis
743
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
743
Mass Spectrometry: Overview
5.0K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.0K
