相关实验视频

Updated: Jun 23, 2025

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

m⁵C甲基化lncRncr3-MeCP2相互作用限制了miR124a启动的神经发生

Jing Zhang¹, Huili Li², Lee A Niswander³

¹Department of Molecular, Cellular, and Developmental Biology. University of Colorado Boulder, Boulder, CO, 80309, USA. jing.i.zhang@colorado.edu.

Nature communications

|June 15, 2024

概括

甲基化lncRNA Rncr3和MeCP2通过调节miR124a在大脑发育过程中的生物发生来控制神经前代细胞的增殖和神经分化.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
发展生物学发展生物学

背景情况:

神经元分化和神经前体细胞 (NEPC/NPC) 扩张对于早期大脑发育至关重要.
非编码RNA在调节这些过程中起着至关重要的作用.
大脑发育中的长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 之间的相互作用是一个活跃的研究领域.

研究的目的:

研究lncRNA Rncr3和microRNA miR124a-1在调节神经生成中的独立和对立的作用.
阐明Rncr3控制NEPC/NPC增殖和miR124a生物发生的机制.
了解MeCP2如何调解Rncr3和miR124a之间的相互作用以协调大脑生长.

主要方法:

在体外和体内使用神经前体细胞进行的研究.
对lncRNA和miRNA的表达和功能的分析.
对Rncr3.3的甲基化分析.
MeCP2 结合测定. 结合测试.
RNA免疫沉 (RIP) 测试用于识别相互作用的蛋白质.

主要成果:

Rncr3和miR124a-1在NEPC/NPC增殖和神经元分化中表现出独立和对立的作用.
Rncr3调节NEPC/NPC的扩散,并控制miR124a.a.的生物发生.
通过MeCP2结合的Rncr3保存表突2/3的细胞因子甲基化,限制miR124a的表达,防止神经元过早分化,并确保正常的大脑生长.
MeCP2结合甲基化Rncr3并通过招募PTBP1来抑制miR124a处理,从而阻止DROSHA-DGCR8的访问.

结论:

lncRNA Rncr3和microRNA miR124a-1是早期大脑发育的关键调节者.
通过MeCP2-介导的miR124a处理的抑制,Rncr3控制神经母细胞扩张和神经元分化之间的平衡.
lncRNA m5C甲基化和MeCP2-依赖的表写体调节对于协调大脑发育至关重要.

更多相关视频

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Published on: March 28, 2022

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...