在ENL中读取 histone β-hydroxybutyrylation,以调节基因转录
Chen Chen1, Cong Chen1, Aiyuan Wang1
1The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, China.
Nucleic acids research
|June 16, 2024
概括
研究人员将ENL识别为基因素β-xybutyrylation (Kbhb) 的读取器,这是将新陈代谢与基因表达联系起来的标记. 这一发现揭示了一种新的表观遗传机制,调节了参与细胞增殖的基因.
科学领域:
- 生物化学 生物化学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 基因突变对调节基因转录至关重要.
- lysine β-hydroxybutyrylation (Kbhb) 是一种新兴的组蛋白标记,将细胞代谢与基因表达联系起来.
- 识别识别Kbhb的蛋白质"读者"对于理解其调节作用至关重要.
研究的目的:
- 为了识别 histone β-hydroxybutyrylation (Kbhb) 的新型蛋白质读取器.
- 阐明Kbhb影响基因转录的分子机制.
- 为了研究Kbhb-reader相互作用在细胞过程中的功能意义.
主要方法:
- 使用多价值光亲缘关系探针开发化学蛋白质学方法.
- 鉴定素H3氨酸9β-xybutyrylation (H3K9bhb) 的结合伙伴.
- 生物化学测试和CUT&Tag分析以验证蛋白质标记相互作用和基因组定位.
主要成果:
- ENL被确定为一种新型读者蛋白,该蛋白特别结合H3K9bhb.
- 在促进子区域内,ENL与H3K9bhb共同定位,调节基因表达.
- 破坏H3K9bhb-ENL相互作用抑制了像MYC这样的增殖驱动基因的表达.
结论:
- 恩尔作为基因素β-xybutyrylation的一个关键效应因子,调节基因转录.
- 这项研究提供了一种化学蛋白质组的策略,用于识别新型基因组标记的读者.
- 这些发现为表观遗传调节和基因素Kbhb的功能作用提供了新的见解.
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