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糖尿病微环境恶化了脂肪介质细胞 stromal 细胞的再生能力

Sara M Ahmed¹, Hoda A Elkhenany^1,2, Toka A Ahmed¹

¹Center of Excellence for Stem Cells and Regenerative Medicine (CESC), Zewail City of Science and Technology, 6th of October City, Sheikh Zayed District, 6th of October City , 12582, Giza, Egypt.

Diabetology & metabolic syndrome

|June 16, 2024

概括

糖尿病疾病会损害脂肪干细胞 (ASC) 的功能,增加氧化应激和亡. 然而,ASCs表现出适应性反应,诱导抗氧化应激基因以抵消有害的糖尿病微环境.

科学领域:

内分泌学和再生医学的研究.
细胞和分子生物学是细胞和分子生物学.

背景情况:

2型糖尿病 (T2DM) 的特点是胰岛素抵抗.
脂肪干细胞 (ASC) 由于其免疫调节和分化能力,对T2DM具有治疗潜力.
糖尿病微环境可能会对ASC的功能产生负面影响.

研究的目的:

研究糖尿病微环境对脂肪干细胞 (ASC) 恒常性和再生潜力的影响.
阐明高血糖症中ASC功能障碍背后的机制.

主要方法:

在糖尿病血清 (DS) 与正常血清 (NS) 中培养的ASC的差异基因表达和蛋白质组分析.
评估ASC亡,氧化应激,DNA修复,表面标记物表达和分化能力.
评估细胞因子和细胞因子受体表达,以确定ASC-微环境交叉对应.

主要成果:

糖尿病血清诱导ASC的显著变化,包括增加的亡,氧化应激和DNA损伤.
在DS中培养的ASCs表现出骨质原,脂肪原和血管原分化受损.
免疫调节潜力受到损害,具有改变的亲和抗炎细胞因子表达.
抗氧化应激基因和蛋白质 (例如SIRT1,TERF1,Clusterin,PKM2) 的上调.

结论:

在糖尿病中,ASCs的再生能力下降,部分原因是氧化应激和炎症.
抗氧化压力因素的升级表明ASC对糖尿病环境的适应性反应.

关键词:

脂肪性干细胞是一种脂肪性干细胞.血管新生的产生.糖尿病是一种糖尿病.糖尿病患者的患者.糖尿病人的血清.代谢活动代谢活动.2 型糖尿病 2 型糖尿病

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...