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长读RNA测序可以探测有机体基因组的普遍转录

Matheus Sanita Lima¹, Douglas Silva Domingues², Alexandre Rossi Paschoal³

¹Department of Biology, Western University, 1151 Richmond Street, London, Ontario N6A 5B7, Canada.

Briefings in functional genomics

|June 17, 2024

概括

有机体基因组,曾经被认为是简单的,显示了多样化的架构和广泛的转录. 长期读取的RNA测序和公共数据为复杂的有机体非编码转录组提供了新的见解.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

在历史上,有机体基因组被认为是 prokaryotic DNA 的简单残余.
最近的有机体基因组学揭示了基因组大小,结构和内容的显著多样性.
有机体基因组被广泛转录,产生大量的RNA类型,但对非编码RNA的理解仍然很差.

研究的目的:

审查湿实验室实验和第二代测序用于器官RNA分析的实用性.
突出第三代 (长读) RNA测序作为有机体转录组学的未来.
倡导在有机体研究中使用公开可用的测序数据.

主要方法:

审查现有的文献和测序数据分析.
探索第二代 (短读) 和第三代 (长读) RNA测序技术.
对NCBI SRA等公共存储库中的数据进行分析,以进行比较研究.

主要成果:

第二代测序有助于识别特定的有机体RNA,特别是非编码的RNA.
第三代RNA测序预示着有机体转录组的更深的分辨率.
在公共存储库中,有足够的数据用于跨类比较器官基因组学研究.

结论:

有机体转录学正在推进新的测序技术和数据可访问性.
公开可用的数据对以有机体为重点的研究具有重大潜力.
专门的有机体基因组学/转录基因组学数据库对于推进该领域的基本和应用影响至关重要.

关键词:

长时间读取的RNA测序.线粒体转录线粒体转录没有编码的RNA.有机体基因组的基因组无处不在的转录.塑体转录转录塑体转录

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