准备上市的U-AST套件:用于同时检测尿路感染和抗生素耐药性的简单,快速,经济有效的解决方案
Maheshawari J Behere1,2, Soumya Haldar1,2
1Analytical and Environmental Science Division & Centralized Instrument Facility, CSIR-Central Salt & Marine Chemicals Research Institute, G.B. Marg, Bhavnagar 364002, India. shaldar@csmcri.res.in.
Analytical methods : advancing methods and applications
|June 17, 2024
概括
一个新的尿路感染抗生素敏感性测试 (U-AST) 套件提供了快速,廉价的细菌感染和抗生素耐药性的检测. 这种简单的颜色变化测试在9小时内提供结果,有助于及时治疗和减少抗生素耐药性的传播.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 微生物学 微生物学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 尿路感染 (UTI) 需要快速诊断和抗生素敏感性测试.
- 目前的诊断方法耗时,需要专门的实验室设备和人员.
- 越来越需要可访问,廉价和快速的尿路感染检测方法,这些方法也可以确定抗生素耐药性.
研究的目的:
- 开发和验证一种新的尿路感染抗生素敏感性测试 (U-AST) 工具包.
- 提供一种快速,简单,廉价的检测尿路感染及其相应抗生素耐药性特征的方法.
- 帮助临床医生处方适当的抗生素,从而改善患者的治疗结果,减轻抗生素耐药性的传播.
主要方法:
- 使用参考细菌菌株 (例如, *Escherichia coli*, *Enterococcus faecalis*) 的U-AST套件的标准化.
- 使用50个具有不同物理和化学性质的临床尿样验证套件.
- 测试细菌对五种主要抗生素的耐药性模式.
主要成果:
- 该U-AST套件显示100%与黄金标准实验室方法一致.
- 该套件通过简单的颜色变化,在最长9小时内识别了细菌污染和抗生素耐药性概况.
- 传统的方法需要36-72小时和专业的专业知识.
结论:
- 开发的U-AST套件为UTI诊断和抗生素敏感性测试提供了一种独特,快速,廉价和用户友好的方法.
- 这种方法消除了对专门实验室设置或专家人员的需求.
- 该U-AST套件有可能显著改善尿路感染管理和打击抗菌素耐药性,目前正在进行商业化.
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