变化的阴道宫微生物群多样性有助于HPV诱导的宫癌通过炎症调节
Yiheng Yang1, Jufan Zhu1, Renqian Feng1
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
PeerJ
|June 17, 2024
概括
阴道微生物群的变化,包括乳酸菌减少和花菌增加,与HPV诱导的宫癌相关. 这些变化可能会影响Th1/Th2细胞因子调节,影响瘤免疫力,并提供新的诊断和预防策略.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 妇科瘤学 妇科瘤学
背景情况:
- 宫癌是全球女性健康的一个重要问题,与人类乳头瘤病毒 (HPV) 感染有着密切联系.
- 阴道微生物群在与HPV相关的宫癌的发展中发挥着作用,可能是通过影响Th1/Th2免疫反应.
- 阴道微生物群调节宫癌免疫微环境的确切机制尚不清楚.
研究的目的:
- 在HPV诱导的宫病变患者中,研究阴道微生物群组成和Th1/Th2细胞因子概况之间的相关性.
- 为了确定与宫癌的进展相关的特定微生物特征.
- 在瘤免疫的背景下,探索阴道微生物群变化的功能影响.
主要方法:
- 收集了99名HPV感染患者的阴道样本,病变程度和对照组不同.
- 在阴道样本上进行细菌DNA测序,以分析微生物群组成.
- 使用ELISA对阴道-宫分泌物中的量化Th1/Th2细胞因子 (IL2,IL12,IL5,IL13,TNFa) 水平进行分析,并分析了与微生物群的相关性.
主要成果:
- 确定了减少的 * Lactobacillus * 和增加的 * Gardenerella * 丰度和由HPV诱导的宫癌之间的显著相关性.
- 预测分析表明,在宫癌患者的阴道微生物群中,ABC载体通路的丰富.
- 观察到特定微生物群变化与对于瘤免疫至关重要的Th1/Th2细胞因子的产生之间的相关性.
结论:
- 阴道微生物群失生可能通过调节Th1/Th2细胞因子反应,导致HPV诱导的宫癌进展.
- 这些发现为阴道微生物组和宫癌发生之间的相互作用提供了新的见解.
- 已识别的微生物和细胞因子标记物具有早期诊断和开发宫癌创新预防策略的潜力.
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