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Updated: Jul 26, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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在测量循环微RNA时进行分析前,分析后和分析后的考虑

Mustapha Zendjabil1,2

  • 1Faculty of Medicine, University of Oran 1 - Ahmed Ben Bella, Oran, Algeria.

Biochemia medica
|June 17, 2024
PubMed
概括

循环的microRNAs (miRNAs) 显示出作为疾病生物标记物的希望,但不一致的结果阻碍了它们的临床使用. 本综述强调了影响miRNA检测的因素,并建议对可靠的非侵入性生物标志物测量进行标准化.

关键词:
生物标志物 生物标志物收集血液 收集血液液体活检活检液体活检微RNAs 是一个微型RNA.预分析阶段 预分析阶段

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...