频段计数不精确 - - 克罗地亚多中心试点研究
Vanja Radišić Biljak1,2,3, Višnja Jureša3,4, Valentina Vidranski3,5
1Department of Medical Laboratory Diagnostics, University Hospital Sveti Duh, Zagreb, Croatia.
Biochemia medica
|June 17, 2024
概括
在克罗地亚实验室中,计数带中性粒细胞的观察者间变异性仍然很高,尽管国际建议将它们与细分中性粒细胞分组在一起. 这种不一致性需要国家一级的行动来标准化白细胞差异分析.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 实验室医学 实验室医学
背景情况:
- 国际血液学标准化委员会 (ICSH) 2015年建议,由于观察者间的高变异性,建议将带中性粒细胞计数为细分中性粒细胞.
- 尽管有建议,许多克罗地亚实验室继续在白细胞 (WBC) 差异中分别报告带中性粒细胞.
- 这种做法可能导致患者的诊断和治疗不一致.
研究的目的:
- 评估克罗地亚实验室在WBC差异内计数带中性粒细胞的观察者间变异程度.
- 评估血液学实验室关于带中性粒细胞计数的当前做法.
- 为潜在的国家标准化工作提供数据.
主要方法:
- 这是一项涉及克罗地亚七家大型医院实验室的多中心研究.
- 通过分析器标记潜在带中性粒细胞的血涂片的分析.
- 所有参与实验室人员评估带中性粒细胞计数.
- 使用变化系数 (CV) 计算观察者之间的可重现性.
主要成果:
- 在计数带中性粒细胞时,观察者间变异性非常高,CVs从15.4%到62.4%不等.
- 分段性中性粒细胞的CVs通常较低,从7.4%到32.2%.
- 没有发现简历与参与医院的员工人数之间的相关性.
结论:
- 该研究证实了克罗地亚实验室中带中性粒细胞计数在观察者之间显著的变异性.
- 显然需要国家一级的行动来规范带中性粒细胞的列举.
- 标准化将提高克罗地亚WBC差异分析的可靠性和一致性.
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