相关实验视频

Updated: Jun 23, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.1K

SPLICER:一个高效的基础编辑工具箱,可以在vivo中实现治疗性外子跳转

Angelo Miskalis, Shraddha Shirguppe, Jackson Winter

    bioRxiv : the preprint server for biology
    |June 17, 2024
    PubMed
    概括

    新的基础编辑工具箱SPLICER通过编辑拼接位置精确地跳过exons. 这项技术通过减少有害的Aβ的形成来治疗阿尔茨海默病.

    更多相关视频

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    7.1K
    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors09:22

    Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

    Published on: February 28, 2021

    5.5K

    相关概念视频

    Base Excision Repair01:54

    Base Excision Repair

    One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
    The first step of...
    22.3K
    Conservative Site-specific Recombination and Phase Variation02:53

    Conservative Site-specific Recombination and Phase Variation

    Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
    The recognition sites for Cre recombinase called LoxP...
    6.0K
    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    50.5K
    RNA Editing02:23

    RNA Editing

    RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
    9.0K
    In-vitro Mutagenesis01:16

    In-vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
    13.9K
    Long-patch Base Excision Repair01:02

    Long-patch Base Excision Repair

    Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
    7.0K