相关实验视频

Updated: Jun 13, 2026

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

无血血液作为全血的潜在替代品,用于转录基因分析

Qingwang Chen¹, Xiaorou Guo¹, Haiyan Wang¹

¹State Key Laboratory of Genetic Engineering, School of Life Sciences and Human Phenome Institute, Shanghai Cancer Center, Fudan University, Shanghai, 200438 China.

Phenomics (Cham, Switzerland)

|June 17, 2024

概括

无等离子体血液 (PFB) 是精密医学中RNA测序 (RNAseq) 的全血 (WB) 的可行替代品. PFB样本与WB具有很高的相似性,使得精确的基因表达分析和免疫学表征成为可能.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

RNA测序 (RNAseq) 对于精准医学至关重要,它利用人类外周血液.
血液样本包括全血 (WB),血,血清和副产品,如无血血液 (PFB) 和无血清血液 (SFB).
对于转录组分析的PFB和SFB的实用性在很大程度上尚未被探索.

研究的目的:

评估PFB和SFB作为转录组分析的替代RNA来源.
为了比较PFB和SFB与WB在RNAseq应用中的适用性.
确定PFB是否可以在研究中成为WB的经济有效替代品.

主要方法:

对WB,PFB和SFB样本进行比较的转录基因分析.
评估蛋白质编码基因表达模式.
不同基因表达分析和免疫学表征.

主要成果:

在基因表达特征上,PFB样本与WB样本具有显著的相似性.
在保持RNA完整性和分析信息方面,PFB的表现优于SFB.
PFB适用于检测差异表达基因和免疫分析.

结论:

在转录基因研究中,PFB可以作为WB的实用和可靠的替代RNA源.
这一发现支持优化血液样本在精密医学研究中的使用.
使用PFB可以提高RNAseq应用的效率和范围.

关键词:

没有血的血液.在RNA测序过程中,RNA测序没有血清的血液.转录组分析转录组分析整体血是全身血液中的一种.

更多相关视频

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Published on: March 20, 2021

相关概念视频

Blood Transfusion

Blood Transfusion

Blood transfusion is a critical medical procedure that saves lives and treats various medical conditions. It involves transferring blood from a donor to a recipient. This process requires a thorough understanding of the ABO blood group system and its associated antigens and antibodies.
Blood Transfusion Overview
A blood transfusion is a medical procedure used to replace blood lost due to injury, surgery, or to treat conditions such as anemia or cancer. During a transfusion, donor blood is...

Bone Marrow Sampling and Transplants

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...