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识别了多ADP-ribose) 聚合酶9 (PARP9) 作为一种强大的抑制剂,用于Mycobacterium tuberculosis感染

Zhenyu Zhu¹, Shufeng Weng¹, Fen Zheng¹

¹State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433 China.

Phenomics (Cham, Switzerland)

|June 17, 2024

概括

在结核病患者中,多分子ADP-ribose聚合酶 (PARP) 发生变化. PARP9作为对抗Mycobacterium感染的抑制剂,减少了细菌的增殖.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
微生物学微生物学

背景情况:

ADP-ribosylation是通过ADP-ribosyltransferases (ARTs) 介导的一个关键的翻译后修饰.
聚基聚合酶 (PARP) 是人类关键的ART,参与宿主-病原体相互作用,但它们在Mycobacterium感染中的作用尚不清楚.

研究的目的:

在Mycobacterium感染期间调查PARP的表达和功能.
阐明PARP9在控制Mycobacterium感染中的作用.

主要方法:

在结核病患者中对PARP表达的生物信息分析.
在受Mycobacterium感染的细胞系中验证PARP表达变化 (THP1,A549).
在Mycobacterium感染细胞中对PARP9缺乏和再表达的功能性研究.

主要成果:

在结核病患者中观察到明显改变的PARP表达,治疗后正常化.
菌根菌感染上调了PARP9,PARP10,PARP12和PARP14的mRNA和蛋白质水平.
PARP9 缺乏增强了 Mycobacterium 感染和细胞内增殖,这被 PARP9 再表达逆转.

结论:

这项研究揭示了在Mycobacterium感染期间PARP表达的动态变化.
PARP9被确定为Mycobacterium感染的显著抑制剂,突出其作为治疗点的潜力.

关键词:

这是Mycobacterium.这种细菌是Mycobacterium smegmatis.聚 (((ADP-ribose) 聚合酶 9 的多种类型聚 ((ADP-ribose) 聚合酶的聚合物.结核病是一种疾病.

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