通过从肠道微生物组检测水平基因转移事件来增强对疾病的洞察力
Shuai Wang1,2, Yiqi Jiang1,2, Lijia Che1,2
1City University of Hong Kong Shenzhen Research Institute, Shenzhen, China.
Nucleic acids research
|June 17, 2024
概括
当地HGT准确地检测了肠道微生物群中的完整水平基因转移 (HGT) 事件. 这种方法揭示了HGT.
科学领域:
- 微生物学和基因组学 微生物学和基因组学
- 生物信息学和计算生物学
背景情况:
- 横向基因转移 (HGT) 在肠道微生物群中普遍存在,并影响人类健康.
- 从元基因组数据准确识别完整的HGT事件,包括断点,是具有挑战性的.
研究的目的:
- 开发一种可靠和快速的方法,LocalHGT,用于从猎枪元基因组数据中检测完整的HGT事件.
- 系统地描述人类肠道微生物群中的HGT事件,并探索它们与疾病的关联.
主要方法:
- 开发和应用LocalHGT工具用于HGT事件检测.
- 使用纳米孔数据和模拟数据集进行验证.
- 对2098个人类肠道微生物样本中HGT事件的分析.
主要成果:
- 在检测完整的HGT事件时,LocalHGT获得了高精度 (99.4%) 和F1得分 (0.99).
- 在HGT断点交叉点上,微同质学得到了显著的丰富.
- HGT事件充当宿主特异性指纹,在人内时间样本中相似性更高.
- HGTs与结直肠癌 (CRC) 通过丁酸盐代谢和急性腹通过石病途径有关.
- 不同的HGT显示出作为疾病生物标志物的潜力,在CRC预测中达到0.87的AUC.
结论:
- 局部HGT提供了一种强大的方法,用于在元基因组数据中识别完整的HGT事件.
- HGT事件提供了对肠道微生物组动态,宿主特异性和疾病关联的见解.
- 对于开发新型的诊断和预测生物标志物来研究CRC等疾病,HGT分析具有前景.
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