相关实验视频
Updated: Jun 23, 2025

12:19
Three-Dimensional 3D Tumor Spheroid Invasion Assay
Published on: May 1, 2015
57.3K
癌细胞抗辐射,整合素αvβ3和甲状腺激素的其他考虑因素
Gennadi V Glinsky1, Aleck Hercbergs2, Shaker A Mousa3,4
1Institute of Engineering on Medicine, University of California, San Diego/LaJolla, CA, USA.
Endocrine research
|June 17, 2024
概括
经过化学修饰的四甲基酸 (CMT) 通过降低PARP9,STAT3和EGFR等关键基因的调节来降低癌细胞的放射电阻. 这种甲状腺激素类似物显示出增强特定抗辐射瘤的放射治疗的前景.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 放射治疗研究 放射治疗研究
背景情况:
- 甲状腺激素类似物和癌细胞辐射抵抗之间存在功能联系.
- 二甲酸 (tetrac) 和其化学修饰的类似物 (CMT) 恢复了抗辐射瘤细胞的辐射敏感性.
- 假设CMT的机制涉及甲状腺激素模拟受体在整合素αvβ3.3.上.
研究的目的:
- 研究CMT对癌细胞辐射敏感性的潜在治疗作用背后的分子机制.
- 分析CMT类似物如何影响与恶性细胞抗辐射相关的基因的表达.
主要方法:
- 在人类质母细胞瘤 (GBM) 和急性髓细胞白血病 (AML) 细胞系上进行了全基因组基因表达特征分析.
- 细胞在体外暴露于CMT类似物的非细胞毒性剂量.
- 对以前与癌症放射电阻相关的特定基因组的基因表达变化进行了分析.
主要成果:
- 在AML (PARP9,PARP15,STAT3) 和GBM (PRKDC,EGFR,CCND1) 细胞系中,CMT治疗显著降低了特定基因三元体的表达.
- 在CMT暴露后,主要患者衍生的GBM细胞中,更广泛的抗辐射相关基因受到影响.
- 观察到的基因表达变化与癌细胞恢复的放射敏感性有关.
结论:
- CMT类似物调节癌细胞抗辐射作用的关键基因的表达.
- 这些发现表明CMT的潜力是作为特定耐辐射瘤的辐射治疗的辅助.
- 需要进行进一步的临床和基因组研究,以确定可能受益于四基剂的患者群体和瘤类型.
相关概念视频
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Activation of Integrins
3.4K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
3.4K
Adaptive Mechanisms in Cancer Cells
5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
Integrins
3.9K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
3.9K
Targeted Cancer Therapies
7.5K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.5K
Synthesis and Regulation of Thyroid Hormones
4.5K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.5K

