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检测和量化携带抗生素耐药性基因的移动遗传元素的结合转移

Virginie Libante¹, Manon Dechêne-Tempier^1,2, Nathalie Leblond-Bourget¹

¹Université de Lorraine, INRAE, DynAMic, Nancy, France.

Methods in molecular biology (Clifton, N.J.)

|June 17, 2024

概括

移动遗传元件 (MGE),就像整合和结合元件 (ICE) 和整合和可调动元件 (IME) 一样,在Streptococcus suis.中传播抗菌素耐药性基因. 评估它们的流动性对于理解抵抗传播至关重要.

科学领域:

微生物学微生物学
遗传学遗传学是一种遗传学.
分子生物学分子生物学

背景情况:

多种药物耐药性是动物感染病原体Streptococcus suis.日益令人担忧的一个问题.
抗微生物耐药性基因通常位于染色体移动遗传元件 (MGE) 上,包括整合和结合元件 (ICE) 和整合和可调动元件 (IME).
复杂的MGE安排,如合体或嵌套的复合元件,阻碍了它们的识别.

研究的目的:

研究Streptococcus suis.中携带抗菌素耐药基因的MGE的移动性.
建立评估通过MGEs转移和传播抗性基因的方法.

主要方法:

选择合适的捐赠者-接受者细菌对进行交配测试.
执行基于PCR的切除试验,以确认遗传元素的循环化.
进行结合测试以量化MGE在菌株之间的转移.

主要成果:

证明了MGEs从Streptococcus suis染色体中切除的能力.
成功评估了菌株之间MGE的结合性转移.
确定了复杂的MGE结构,使分析复杂化.

结论:

MGEs,特别是ICE和IME,在Streptococcus suis种群内和之间传播抗菌素耐药性基因方面发挥着重要作用.
了解MGE的流动性对于控制在One Health环境中的耐药性传播至关重要.
评估MGE流动性的标准化方法对于监测和干预策略至关重要.

关键词:

抗生素耐药性抗生素耐药性结合结合结合结合切除切除的方法是切除.冰冰冰冰冰冰 IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME IME 移动遗传元素移动遗传元素这是一种Streptococcus suis.

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