生物炭调节细胞内电子转移以减少酸盐在去化无氧甲氧化古物中
Pan-Long Lv1, Chuan Jia1, Chi-Hang Wei1
1Key Lab of Environmental Engineering, Shaanxi Province, Xi'an University of Architecture and Technology, No.13 Yanta Road, Xi'an 710055, China.
Bioresource technology
|June 17, 2024
概括
生物炭增强了无氧甲氧化 (DAMO) 古代生物的脱活动,改善了去除和甲缓解. 它促进微生物聚合和电子转移,有助于酸盐的减少.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 生物地质化学生物地质化学
背景情况:
- 脱无氧甲氧化 (DAMO) 古代生物对于同时去除和缓解甲至关重要.
- 达摩古生物的有限代谢活动限制了它们的工程应用.
研究的目的:
- 研究生物炭在增强DAMO微生物的代谢活性和酸盐降解能力方面的潜力.
- 阐明生物炭影响DAMO古物种的机制.
主要方法:
- 将粉生物炭添加到DAMO古生物学文化中.
- 测量酸盐和酸盐的降解率.
- 扫描电子显微镜 (SEM) 和光激发发射矩阵 (EEM) 分析.
- 评估生物膜氧化还原能力,导电性和电子转移系统活动.
- 关键代谢和运输蛋白质的基因表达分析.
主要成果:
- 粉生物炭显著提高了酸盐减少率的2.85倍.
- 生物炭促进了微生物聚合和细胞外聚合物质 (EPS) 的分泌.
- 生物炭增强了生物膜氧化还原能力,导电性和电子转移系统活动的1.65倍.
- 参与电子运输 (Hdr,MHC,Rnf) 和膜运输 (BBP,ABC,NDH) 的关键基因被显著上调.
结论:
- 生物炭有效地增强了DAMO古生物的代谢活性,改善了酸盐的减少.
- 生物炭有助于从逆甲生成到膜的电子转移,以减少酸盐.
- 生物炭显示出优化基于DAMO的生物修复策略以控制和甲的前景.
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