帕尔3/巴索卡结合了NICD,并在Drosophila发育过程中促进了痕信号传递
Jun Wu1, Neeta Bala Tannan1, Linh T Vuong1
1Dept. of Cell, Developmental, and Regenerative Biology, Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY, 10029, USA.
巴祖卡 (Baz/Par3) 蛋白直接调节Notch信号传递,这对于基因激活至关重要. 这种极性蛋白独立于aPKC的功能,与NICD相互作用,促进Notch通路活性.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 基地座 (Baz/Par3) 基因对于动物的不对称细胞分裂至关重要.
- Baz/Par3与Par6和非典型蛋白激酶C (aPKC) 形成复合体,参与在上皮细胞中建立细胞极性.
研究的目的:
- 调查 Baz/Par3 在 Notch 信号通路中的潜在新作用.
- 为了确定Baz/Par3是否调节Notch信号活动和目标基因转录.
主要方法:
- 利用Drosophila melanogaster模型进行翅膀和眼睛发育研究.
- 采用了Baz/Par3和aPKC的淘汰实验.
- 分析了转基因的诺奇结构,并进行了生化相互作用测试.
主要成果:
- Baz/Par3对于Notch信号活动和Notch目标基因的最佳转录激活至关重要.
- 在Notch信号中,Baz/Par3函数是独立于aPKC的.
- Baz/Par3在Notch裂变的下游和Su (H) /CSL转录因子活动的上游作用.
- 在Notch细胞内域 (NICD) 和Baz/Par3之间展示了直接的生化相互作用.
结论:
- Baz/Par3充当了Notch信号的积极调节者.
- Baz/Par3与NICD直接相互作用,增强其在Su(H) /CSL结合之前的活动.
- 这项研究定义了极性蛋白 Baz/Par3 在调节 Notch 信号的新功能.
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