从白树中共同生产可发酵糖和高活性素,通过轻度预处理与二乙烯糖醇和化
Song Tang1, Shen-Ao Yuan2, Yequan Sheng2
1College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu, Anhui 241000, China; Biomass Group, College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu 210031, China.
International journal of biological macromolecules
|June 17, 2024
概括
这项研究使用乙烯基甘醇和CrCl3对白树进行了轻度预处理,实现了高度的脱和纤维素回收. 这一过程增强了酶性水解以增加葡萄糖产量,并产生有价值的红素.
科学领域:
- 生物质预处理 生物质预处理
- 纤维素的价值化 纤维素的价值化
- 绿色化学 绿色化学
背景情况:
- 将纤维素生物质有效转化为生物燃料和生物化学品需要有效的预处理策略.
- 传统方法往往会产生抑制的副产品和恶劣的条件,限制可扩展性.
- 开发温和高效的预处理方法对于可持续的生物质利用至关重要.
研究的目的:
- 研究一种新的轻度预处理方法,使用乙烯基醇和CrCl3.Cl进行白树的预处理.
- 为了提高纤维素可用于酶性水解的可访问性.
- 探索可发酵糖和高质量的红素的联合生产.
主要方法:
- 欧卡利普斯用乙烯基醇和0.02mol/L CrCl3进行预处理,持续10分钟.
- 预处理和未处理的白树的酶性水解.
- 使用各种分析技术对预处理生物质和提取的红素进行表征.
- 对酶吸附和葡萄糖产量的评估.
主要成果:
- 通过最小的发酵抑制剂实现了91%的脱和98%的纤维素回收.
- 细胞质可访问性和吸附率增加了1.6倍.
- 获得了7.9倍高的葡萄糖产量,达到Tween 80的62 g/L.
- 与商业性木质素相比,de-lignin显示出优越的性能.
结论:
- 乙烯基醇和CrCl3的预处理是一种温和而有效的 lignocellulose 价值化方法.
- 这种方法促进了可发酵糖的高产量,并产生高活性素.
- 它为生物质的有价值产品的联合生产提供了一个有前途的途径.
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