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使用Deinococcus deserti的氨基糖糖酶提高了myricetinα-triglucoside的有效和新型生物合成和可溶性

Joong-Ki Im¹, Dong-Ho Seo¹, Jae Sik Yu²

¹Department of Food Science & Biotechnology, Carbohydrate Bioproduct Research Center, Sejong University, Seoul 05006, Republic of Korea.

International journal of biological macromolecules

|June 17, 2024

概括

这项研究使用Deinococcus deserti amylosucrase (DdAS) 生物合成了一种新的myricetin (MYR) α-triglucoside (MYRαG3). MYRαG3保留了抗氧化活性,并显示出大大提高了水溶性,增强了其作为功能性食品材料的潜力.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
食品科学食品科学食品科学

背景情况:

米瑞 (MYR) 具有显著的抗氧化特性,但由于稳定性和水溶性差,在功能性食品应用中受到限制.
氨基糖酶 (ASase) 酶可以合成黄醇α-糖化物,从而有可能改善像MYR这样的化合物的特性.

研究的目的:

使用特定的氨基糖酶 (DdAS) 来生物合成一种新型的myricetinα-triglucoside (MYRαG3).
为了描述合成的化合物,并评估其与母药myricetin相比的特性.

主要方法:

使用Deinococcus deserti (DdAS) 的ASase用于MYR和糖糖的MYRαG3的酶合成.
采用比较同质分析和计算模拟来了解DDAS酶特征.
使用NMR和LC-MS确定了MYRαG3的结构.
评估了MYRαG3.3的DPPH激素清除活性和水溶性.

主要成果:

DdAS有效合成了MYRαG3,其转化率为67.4%.
这种结构被确定为MYR 4'-O-4′′,6′′-tri-O-α-D-glucopyranoside.
MYRαG3表现出与MYR.相似的DPPH激素清除活性 (49%),与MYR.相似.
MYRαG3的水溶性从0.03微克/毫升大幅增加到511.5毫克/毫升.

结论:

DdAS在生物合成一种新型MYRα-triglucoside (MYRαG3) 中是有效的.
在保持抗氧化能力的同时,MYRαG3表现出增强的水溶性.
这种新型化合物有望成为食品应用中改进的功能材料.

关键词:

氨基糖糖酶的氨基糖酶.迪诺科克沙漠菌 (Deinococcus deserti) 是一种植物.米瑞丁α-三糖化物

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