β-arrestins 是支架蛋白质,需要用于Shh介导的轴指导
Rachelle Sauvé1,2, Steves Morin1, Patricia T Yam3
1Montreal Clinical Research Institute (IRCM), Montreal, Quebec H2W 1R7, Canada.
概括
β-arrestins充当关键的支架,将Smo和Src家族激酶 (SFKs) 连接起来,以调解Sonic hedgehog (Shh) 引导的轴突发育. 这项研究揭示了它们在Shh介导的辅助性轴突指导中的重要作用.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 在神经系统发育过程中,Sonic hedgehog (Shh) 对于指导 commissural 轴突至关重要.
- Shh信号传递涉及受体Boc,Smo和Src家族激酶 (SFKs).
- 对于Shh介导的轴突引导,SFK激活是必要的.
研究的目的:
- 为了研究β-arrestins在Shh介导的辅助性轴突指导中的作用.
- 为了确定β-arrestins是否作为链接Smo和SFKs的架构蛋白质起作用.
主要方法:
- 研究了大鼠 commissural神经元中的β-arrestin表达.
- 使用β-止素倒置和主导阴性突变.
- 在体内进行了轴突转向测试,并分析了胚胎脊髓.
主要成果:
- β-阿雷斯与Smo和SFK形成三方复合体,这对复合体形成至关重要.
- β-arrestin knockdown 抑制了 Shh 诱导的 Src 酸化,并阻断了 Shh 介导的轴突吸引.
- 主导阴性β-arrestins破坏了Smo内部化或SFK相互作用,损害了轴突引导.
结论:
- β-arrestins是基本的支架蛋白,在Shh信号中将Smo连接到SFKs.
- 在神经系统发育过程中,β-arrestins对于Shh介导的辅助性轴突指导是不可或缺的.
关键词:
Src 基因酶家族中的基因酶.轴突指导指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导轴突指导导轴突指导导轴突指导轴突指导轴指导轴指导轴这是一种β-arrestin.委托式神经元是一个神经元.刺是一种刺,刺是一种刺.相关概念视频
Assembly of Signaling Complexes
5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
Amplifying Signals via Enzymatic Cascade
8.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.5K
Mechanism of Lamellipodia Formation
2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cell Polarization by Rho Proteins
2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K


