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使用光成像核染色 (FINS) 算法进行图像分析

Laura R Bramwell1, Jack Spencer2, Ryan Frankum1

  • 1RNA-Mediated Mechanisms of Disease Group, Faculty of Life Sciences, Institute of Clinical and Biomedical Sciences, University of Exeter, Exeter, UK.

Journal of imaging informatics in medicine
|June 17, 2024
PubMed
概括

研究人员开发了核染色光成像 (FINS) 算法,以量化免疫细胞化学图像中的光信号. 这种开源工具简化了细胞分析,与手动计数相比,提供一致的结果,主观性和时间减少.

关键词:
自动细胞计数 自动细胞计数光成像成像的使用方法在 Ki6767 的位置上.核污染物是核污染物的一种.γH2AXX 的时间.

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...