Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

In preprints - can a signaling snapshot reveal the fate of a cell?

Development (Cambridge, England)·2026
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

K63-linked ubiquitylation of S2P-RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks.

Nucleic acids research·2026
Same author

Mature Tertiary Lymphoid Structures in Breast Cancers Are Associated With Antitumor Immunity and Better Prognosis.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

Morphogen Gradients as Drivers of Mosaicism During Early Human Development.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same author

Programmed Death Ligand-1 Testing in Triple-Negative Breast Cancer: National Practice and Interlaboratory Variation in the Netherlands.

Laboratory investigation; a journal of technical methods and pathology·2026

相关实验视频

Updated: Jun 23, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K

通过scEdU-seqq量化单细胞中的DNA复制速度.

Jeroen van den Berg1, Vincent van Batenburg2, Christoph Geisenberger2,3

  • 1Oncode Institute, Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Utrecht, The Netherlands. j.berg@hubrecht.eu.

Nature methods
|June 17, 2024
PubMed
概括

在细胞周期的S阶段,DNA复制速度加快. 转录诱导的DNA损伤限制了早期的复制速度,而在较少转录的区域的后期复制速度会加快.

更多相关视频

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.5K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.7K

相关实验视频

Last Updated: Jun 23, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.8K
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

22.5K
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

18.7K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 细胞生物学 细胞生物学

背景情况:

  • DNA复制对于细胞分裂至关重要,涉及数千个复制分叉.
  • 复制速率可能受到表观遗传因素和细胞类型的影响.
  • 精确测量DNA复制速度对于理解基因组复制至关重要.

研究的目的:

  • 开发和应用一种测量单细胞DNA复制速度的方法.
  • 为了研究整个S阶段DNA复制速率的动态.
  • 确定影响DNA复制加速的因素.

主要方法:

  • 单细胞5-乙烯基-2'-脱氧氨酸序列 (seqEdU-seq) 的发展.
  • 使用seqEdU-seq.q.检测新生的复制DNA.
  • 应用遗传和药理学扰动来研究复制动力学.

主要成果:

  • 在S阶段,DNA复制速度不是恒定的,并且在S阶段增加.
  • 转录诱导的DNA损伤限制了S阶段早期的复制速度.
  • 在S阶段晚期,复制加速,因为复制的区域较少转录.

结论:

  • 复制分叉速度在细胞周期期间受到调节.
  • 转录相关的DNA损伤是限制复制速度的关键因素.
  • 了解复制动力学对于基因组稳定性和细胞增殖至关重要.