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人体皮肤纤维细胞衍生的分泌蛋白调节神经恢复:一种生物信息学方法

Sang Bum Suh¹, Ji Youn Suh¹, Hyeri Lee¹

¹BNV Biolab, Seoul, South Korea.

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

|June 18, 2024

概括

皮肤纤维细胞受条件介质 (DFCM) 含有分泌蛋白质,形成在伤口修复期间神经恢复至关重要的网络. 这些蛋白质在组织再生和神经修复过程中起着关键作用.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

人体皮肤纤维细胞分泌蛋白质,对伤口修复和组织再生至关重要.
了解这些分泌的蛋白质对于推进再生医学和治疗策略至关重要.

研究的目的:

识别和描述皮肤纤维细胞受条件介质 (DFCM) 中的分泌蛋白质,这些蛋白质参与神经恢复.
分析这些已识别的DFCM蛋白质的蛋白质-蛋白质相互作用网络.

主要方法:

量化液体染色学-双重质谱法被用来识别DFCM中的337种蛋白质.
生物信息选择确定了可能调节神经恢复的蛋白质.
对57种选择的DFCM蛋白进行了蛋白质-蛋白质相互作用网络分析和基因本体学分类.

主要成果:

57个DFCM蛋白被分为各种功能组,包括蛋白结合活性调节器,细胞骨蛋白和细胞外基质蛋白.
蛋白质与蛋白质相互作用网络分析揭示了这些57种DFCM蛋白质之间的显著相互作用.
鉴定的蛋白质在神经恢复的多个阶段起作用.

结论:

DFCM含有众多的分泌蛋白质,这些蛋白质建立了重要的蛋白质-蛋白质相互作用网络,对神经恢复至关重要.
这些DFCM蛋白质是伤口愈合过程中神经修复的各个阶段的关键调节者.
这项研究强调了DFCM蛋白在增强神经再生方面的治疗潜力.

关键词:

生物信息学是一种生物信息学.皮肤纤维细胞调节的介质.人类皮肤纤维细胞.神经恢复恢复神经恢复神经发生神经发生.蛋白质组分析蛋白质组分析分泌蛋白质是分泌的蛋白质.

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