三脚身体标记和功能分子探针:合成,计算调查和多光子光终身成像显微镜的探索
Marina Lledos1, David G Calatayud2, Fernando Cortezon-Tamarit1
1Department of Chemistry, University of Bath, Bath, BA2 7AY, U.K.
Chemistry (Weinheim an der Bergstrasse, Germany)
|June 18, 2024
概括
有三脚核的新型体型光体被合成用于活细胞成像. 这些探测器使得有针对性的生物结合成为可能,并为先进的纳米材料开发提供了潜力.
科学领域:
- 有机化学 有机化学
- 材料科学 材料科学 材料科学
- 生物医学成像技术 生物医学成像技术
背景情况:
- 开发先进的光探针对于体外测试和活细胞成像至关重要.
- 身体染料是广泛使用的光剂,但需要新的衍生品来增强功能.
- 三脚结构为分子设计提供了独特的特性,并具有纳米材料集成的潜力.
研究的目的:
- 为了合成和表征具有三脚芳香核心的新型BODIPY衍生物.
- 评估它们作为光探针在体外测试和活细胞成像中的潜力.
- 探索它们对混合纳米材料和向生物结合物的功能化.
主要方法:
- 合成的BODIPY衍生物的光谱表征.
- 使用密度函数理论 (DFT) 和时间依赖 DFT (TD-DFT) 进行构造和光物理预测的计算研究.
- 在体外和活细胞成像实验中使用单光子和双光子激发技术,包括2D光终身成像 (2P FLIM).
主要成果:
- 新型三脚体 BODIPY衍生物的成功合成和光谱表征.
- 作为光探针用于体外测试和活细胞成像的潜在潜力.
- 在前列腺癌细胞中通过生物化和细胞吸收和分化成像对向生物结合物的概念验证.
结论:
- 新型三脚体的BODIPY衍生物有望为先进的生物应用提供光体.
- 这些化合物为混合纳米材料和向分子探针提供了功能化的多功能途径.
- 该研究成功地在活癌细胞中成像了这些探针的细胞行为,展示了它们的实用性.
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