通过整合Liriodendron种群中的Pan-transcriptome,GWAS和eQTL分析,揭示了与叶子形态相关的基因
Hainan Wu1,2, Xiao Liu1,2, Yaxian Zong1,2
1State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing, China.
Physiologia plantarum
|June 18, 2024
概括
研究人员通过分析种群转录组和整合基因组关联研究,发现了控制利里奥登德龙叶形状和大小的关键基因. 这揭示了叶子形态发生的分子基础,并有助于种植装饰树.
科学领域:
- 植物生物学 植物生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 叶子形态对于植物发育至关重要,但其遗传基础复杂.
- 柳叶树因其独特的叶形而受到重视,但其形态发生的潜在分子机制尚不清楚.
研究的目的:
- 为了阐明Liriodendron中叶子形态发生的分子机制.
- 识别与叶子大小和形状变异相关的候选基因和调节区域.
主要方法:
- 从81个利里奥登德龙的加入中构建了一个种群级的泛转录组.
- 综合的全基因组关联研究 (GWAS),表达量化特征位点 (eQTL) 和全转录组关联研究 (TWAS).
- 进行了共同表达分析,eQTL和链接映射.
主要成果:
- 通过GWAS通过叶子大小 (18) 和叶子形状 (17) 确定了重要的等位基位.
- 通过TWAS发现了16个与叶子形态相关的候选基因.
- 确定了两个监管热点区域 (hot88和hot758),并确定Lchi_4g10795作为利里奥登德龙叶子发育的关键调节器.
结论:
- Lchi_4g10795通过 cis-eQTL 调节自身表达,影响下游基因控制 Liriodendron 叶子形态.
- 这项研究增强了对Liriodendron叶子形态发生的理解,并为分子育种提供了基础.
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