储存维生素C和维生素E的年轻和老红细胞在添加剂溶液-7-7中的不同反应
Masannagari Pallavi1, Vani Rajashekaraiah1
1Department of Biotechnology and Genetics, School of Sciences, JAIN (Deemed-to-be University), Bangalore, India.
将维生素C和维生素E添加到储存的红细胞 (红细胞) 中,有助于维持它们的健康,特别是在老细胞中. 这种抗氧化剂组合可以在储存期间对抗氧化应激.
科学领域:
- 生物化学 生物化学
- 血液学 血液学 血液学
- 老年学是指老年学的学科.
背景情况:
- 氧化应激 (OS) 显著影响红细胞完整性,主要是由于老化和储存.
- 已知抗氧化剂可以减轻OS,这表明在维护红细胞功能方面可能发挥作用.
研究的目的:
- 评估维生素C和维生素E在储存期间对年轻和老红细胞中抵消OS的疗效.
- 分析这些抗氧化剂对不同红细胞亚群中的各种OS和衰老标志物的影响.
主要方法:
- 红细胞被分为年轻和老的子群体使用Percoll方法.
- 亚种群在4°C下存储21天,在添加剂溶液-7 (AS-7) 或AS-7中与维生素C和E (VC+VE) 添加.
- 在多个时间点评估了氧化应激,抗氧化酶活性和衰老标志物.
主要成果:
- 年轻的红细胞在整个储存过程中与老的红细胞相比,氧化变化较少.
- 虽然抗氧化酶的活性有所不同,但维生素C和维生素E有助于保持老红细胞的氧化还原平衡.
- 晚期氧化蛋白产物和超氧化物在老细胞中增加,但在VC+VE组中,马隆迪化物水平下降.
结论:
- 红细胞亚群对储存和氧化应激有不同的反应.
- 联合维生素C和E补充剂有效地保护了老年红细胞的氧化还原平衡.
- 针对红细胞亚种群的量身定制策略对于有效的血液储存实践至关重要.
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