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富有缺陷的转移性MoS促进巨细胞在乳腺癌中的重编程:临床前景

Mingyue Cui¹, Lulu Qian¹, Ke Lu²

¹Suzhou Key Laboratory of Nanotechnology and Biomedicine, Institute of Functional Nano & Soft Materials & Collaborative Innovation Center of Suzhou Nano Science and Technology (NANO-CIC), Soochow University, Suzhou, 215123, China.

Small (Weinheim an der Bergstrasse, Germany)

|June 18, 2024

概括

富含缺陷的1T2H-二硫化物 (MoS2) 纳米酶有效地再极化瘤相关巨细胞 (TAM) 并杀死癌细胞. 这种新的方法显示了人类乳腺癌免疫治疗的前景.

科学领域:

生物医学工程生物医学工程
材料科学材料科学材料科学
癌症研究癌症研究

背景情况:

瘤相关巨细胞 (TAMs) 是固体瘤免疫抑制和抗原清除的关键调节者.
在临床前模型中,二硫化 (MoS2) 纳米酶在癌症免疫治疗中表现出类似酶的活性.
通过反应性氧物种在临床环境中工程TAM的两极分化仍然是一个挑战.

研究的目的:

为人类乳腺癌免疫疗法设计和合成缺陷丰富的转移稳定的1T2H-MoS2纳米酶.
调查1T2H-MoS2.2.的增强的过氧化酶类活性和基因生成能力.
评估1T2H-MoS2在反极化TAM和诱导癌细胞亡方面的疗效.

主要方法:

合成1T2H-MoS2纳米酶通过减少和相变化在溶液中.
对MoS2纳米酶的表征,包括对过氧化酶类活性和硫空位丰度的评估.
在人乳癌样本中对1T2H-MoS2进行TAM再极化,基生成和癌细胞亡的体外和体外评估.

主要成果:

与商业MoS2.2相比,合成的1T2H-MoS2纳米酶显示过氧化酶类活性增强了约12倍.
在1T2H-MoS2中丰富的硫空缺有助于电荷再分配和增强的催化活性.
1T2H-MoS2有效地产生了细胞外基,将TAM重新极化为类似M1的表型,并直接杀死癌细胞.
人类乳腺癌样本的ex vivo研究显示,1T2H-MoS2的癌细胞亡率与多克索鲁比相比高3.4倍.

结论:

富含缺陷的1T2H-MoS2纳米酶通过调节瘤微环境,为癌症免疫疗法提供了一个有前途的平台.
增强的催化活性和1T2H-MoS2的根基生成使得有效的TAM再极化和直接杀死癌细胞.
在ex vivo人类样本中证明的临床可行性突显了1T2H-MoS2在乳腺癌治疗中的治疗潜力.

关键词:

临床癌症治疗临床癌症治疗巨细胞正在重新编程.纳米酶是一种纳米酶.具有类似过氧酶的活性.阶段转换的相位转换.

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