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使用标准化方法重新定义组织细胞计数:勒文肠道计数协议.

Matthias Ceulemans1, Pauline Huyghe1, Gert De Hertogh2

  • 1Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Chronic Diseases and Metabolism (ChroMeta), Katholieke Universiteit Leuven, Leuven, Belgium.

Clinical and translational gastroenterology
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一种新的软件方法提高了计数十二指甲状腺色素和巨细胞的准确性. 这种标准化的方法有助于通过建立异常细胞透的新值来诊断eosinophilic胃肠道疾病.

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科学领域:

  • 胃肠病学 胃肠病学
  • 组织病理学 组织病理学
  • 数字病理学数字病理学

背景情况:

  • 乙酸性胃肠道疾病的诊断依赖于乙酸细胞计数,但对于十二指肠缺乏标准化值.
  • 方法标准化对于准确的诊断和患者管理至关重要.

研究的目的:

  • 开发一个标准化的工作流程,用于十二指甲细胞量化.
  • 在健康个体中估计正常的十二指甲状腺胆固醇和巨细胞计数.

主要方法:

  • 开发了一种基于软件的组织细胞量化方法,并应用于数字化H&E染色的十二指肠片.
  • 在软件和显微方法之间进行了对比,在独立的队列中验证了可重现性.
  • 与免疫组织化学进行了比较,并且在健康对照组中确定了氨酸和巨细胞的正常范围.

主要成果:

  • 与显微镜 (ICC=0.796) 相比,基于软件的量化显示出显著改善的可重复性 (ICC=0.909).
  • 十二指甲状腺埃索诺菲尔在密室周围的数量比维里尔更多.
  • 在健康对照组中确定了正常范围:平均乙氨基基细胞228.8 / mm2,巨细胞419.5 / mm2 (第95个百分位:乙氨基细胞402.8 / mm2,巨细胞707.6 / mm2).

结论:

  • 开发和验证了一种基于软件的标准化方法,用于十二指甲状腺组织细胞量化.
  • 这种方法可以对各种粘膜细胞类型进行概括.
  • 对于异常的十二指管透,确定了400个乙氨基基/mm2和700个巨细胞/mm2的值.