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气候变化和植物根系球微生物组:一个体验式课程嵌入式研究项目

James A Parejko¹

¹Department of Biology, Gustavus Adolphus College, Saint Peter, Minnesota, USA.

Journal of microbiology & biology education

|June 18, 2024

概括

未来的科学家需要分子和数据技能来应对气候变化. 这个课程嵌入式项目使用DNA测序来研究干旱.

科学领域:

微生物学微生物学
植物科学植物科学
气候变化研究气候变化研究

背景情况:

气候变化带来了持续的挑战,需要科学家熟练的分子技术和数据分析.
了解植物微生物相互作用对于开发适应气候变化的植物至关重要.
下一代测序和生物信息学是现代生物研究的重要工具.

研究的目的:

为气候变化研究准备有分子技术和数据分析实践经验的学生.
为了研究干旱压力的影响根茎球微生物群的Brassica rapa.
在模拟干旱条件下将元数据与微生物多样性变化相关联.

主要方法:

一个课程嵌入的研究项目,利用快速植物快速循环 *Brassica rapa*.
模拟干旱条件适用于使用廉价温室供应品种植的植物.
16S rRNA V3/V4 Illumina测序用于微生物组的分析.
使用QIIME 2微生物组生物信息平台进行数据分析.

主要成果:

鉴定了根系球微生物多样性的变化,以应对模拟干旱.
证明了使用可访问方法研究宿主微生物相互作用的可行性.
为学生提供了下一代测序数据分析的实践经验.

结论:

课程内嵌的研究项目有效地培养学生对气候变化生物学的基本技能.
模拟干旱显著影响了植物根系球微生物群.
该项目为微生物生态学和植物科学领域的可访问,基于调查的学习提供了一个模型.

关键词:

在 QIIME 2 中,QIIME 2 是一个简单的表达式.气候变化气候变化气候变化干旱干旱干旱干旱主体-微生物相互作用微生物组就是微生物组.植物研究研究植物研究根系球 (Rhizosphere) 是一个根系球.

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