瑞林B通过阻断PI3K/AKT信号通路来抑制子宫癌的发展:网络药理学分析和实验验证
Yinwen Hong1, Xueliang Sun2, Lijuan Lu3
1Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.
Applied biochemistry and biotechnology
|June 18, 2024
概括
瑞林B (LB) 有效地抑制了宫癌细胞的增殖,并诱导了细胞亡. 这种天然化合物还通过向PI3K/AKT通路来减少癌细胞的入侵和迁移.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 瑞林B (LB) 是中国龙血的一种成分,在胃癌和肝癌中表现出抗癌特性.
- 之前的研究表明,LB在心脏纤维化,脑缺血/再输和骨质疏松症中的好处.
- 在宫癌中LB的治疗潜力以前没有被研究过.
研究的目的:
- 为了研究Loureirin B (LB) 对宫癌细胞的抗癌作用.
- 阐明LB在宫癌治疗中的作用的基本机制.
- 探索LB对HeLa细胞活力,增殖,细胞周期,细胞亡,迁移和入侵的影响.
主要方法:
- 细胞计数工具-8 (CCK-8) 测试细胞活力.
- 殖民地形成试验对繁殖的测试.
- 流细胞计和西部斑点用于细胞周期和细胞亡分析.
- 斯克拉奇和特朗斯韦尔对迁移和入侵进行了分析.
- 网络药理学用于途径预测和体外验证.
主要成果:
- LB显著降低了HeLa细胞的活力,增殖和殖民地形成.
- 通过调节Bax和Bcl-2表达,LB诱导细胞循环停止和细胞亡的增加.
- 通过降低MMP-2和MMP-9水平,LB抑制了HeLa细胞的迁移和入侵.
- 网络药理学确定PI3K/AKT通路作为一个关键目标,通过PI3K激动剂实验验验证.
结论:
- 瑞林B显示出对宫癌细胞具有显著的抗癌活性.
- LB抑制了增殖,诱导了亡,并减少了宫癌细胞的入侵和迁移.
- LB的抗癌作用通过抑制PI3K/AKT信号通路而得到介导.
相关概念视频
02:34Inhibition of Cdk Activity
4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
02:57Targeted Cancer Therapies
7.5K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.5K
01:22PI3K/mTOR/AKT Signaling Pathway
3.5K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.5K
02:59Cancer Prevention
6.1K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
6.1K