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相关概念视频

Coat Assembly and GTPases01:33

Coat Assembly and GTPases

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Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
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Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

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Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
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Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
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相关实验视频

Updated: Jun 23, 2025

Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
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Leep2A和Leep2B作为RasGAP复合体起作用,以调节巨细胞形成.

Xiaoting Chao1,2, Yihong Yang1, Weibin Gong1

  • 1Key Laboratory of Biomacromolecules (CAS), National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

The Journal of cell biology
|June 18, 2024
PubMed
概括

研究人员发现了Leep2,一个Ras GTPase激活蛋白 (GAP) 复合体,该复合体对于调节巨型皮诺细胞形成至关重要,这是一种用于大量吸收液体的细胞过程. 通过Leep2微调Ras活动对于适当的巨细胞形成至关重要.

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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子信号传递是分子信号传递.
  • 生物化学 生物化学

背景情况:

  • 巨型皮诺细胞体是一种保存的细胞过程,用于大量吸收液体,对于营养获取和环境感知至关重要.
  • 拉斯GTPases是宏皮诺细胞体的中央调节者,但它们的上游调节和时空控制仍然不清楚.
  • 了解控制Ras信号在巨细胞细胞形成中的分子机制对于破译细胞吸收过程至关重要.

研究的目的:

  • 在模型生物Dictyostelium中使用基于蛋白质学的方法识别巨细胞瘤的新型调节者.
  • 阐明已识别的复合体在巨体形成期间Ras GTPase活动的时空调节中的作用.
  • 研究该复合体及其目标Ras GTPases的调制如何影响巨细胞活动.

主要方法:

  • 在Dictyostelium中进行基于蛋白质基的查,以确定参与巨细胞调节的蛋白质.
  • 生物化学试验描述了Leep2复合体作为Ras GTPase激活蛋白 (GAP) 的功能.
  • 基因操纵 (删除,过度表达) 的Leep2和目标RasGTPases,以评估它们对巨型皮诺细胞瘤的影响.

主要成果:

  • 鉴定了Leep2 (由Leep2A和Leep2B组成) 作为一种新的RasGAP复合体,对巨细胞细胞形成至关重要.
  • Leep2复合体的定位以形成巨细胞杯和新生的巨细胞体.
  • 证明Leep2调节了三种Ras家族小GTPase的活性,从而调节了巨细胞形成.
  • 在去除或过度表达Leep2或破坏/持续激活目标Ras GTPases时观察到巨型皮诺细胞形成的损伤.

结论:

  • 利普2复合体在巨细胞结合过程中Ras信号的时空调节中起着至关重要的作用.
  • 微调Ras GTPase活动对于高效和可控的巨类细胞形成至关重要.
  • 这项研究揭示了一种关键的调节机制,它控制了细胞的大量液体吸收.