CBP60b类蛋白质是免疫中介的原型转录因子
Lu-Shen Li1, Yan-Yan Yang2, Yun-Xia Chen2
1Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tian'jin 300071, China.
Plant physiology
|June 18, 2024
概括
卡尔莫杜林 (CaM) 结合蛋白60 (CBP60) 类TFs,特别是CBP60b,对于植物免疫是至关重要的. 这些转录因子在植物进化过程中被保存,调节防御反应.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 转录因子 转录因子
背景情况:
- 转录重编程对于植物免疫反应至关重要.
- 素 (CaM) 结合蛋白60 (CBP60) 转录因子 (TFs) 家族在Arabidopsis thaliana中,包括CBP60g,SARD1,CBP60a和CBP60b,在免疫中发挥关键和独特的作用.
- 其他CBP60家族成员的功能在很大程度上仍未被描述.
研究的目的:
- 为了研究非特征CBP60成员 (CBP60c-f) 在Arabidopsis免疫力中的作用.
- 确定CBP60b分类在植物防御途径中的功能性保护和进化意义.
主要方法:
- 对阿拉比多普西斯CBP60c-f突变的遗传分析.
- 免疫相关基因的表达分析.
- 使用其他植物物种 (番茄,黄瓜,Physcomitrium patens,Selaginella moellendorffii) 的同源基因进行功能补充分析.
主要成果:
- 阿拉比多普西斯CBP60c-f在免疫调节方面与CBP60b表现出冗余的作用,CBP60b驱动的表达补偿了CBP60b的损失.
- CBP60g和SARD1与CBP60b是不可互换的,这表明CBP60家族中的类特定功能.
- CBP60b类TFs的功能取决于它们的DNA结合和CaM结合域,将它们与信号通路联系起来.
- 早期陆地植物 (P. patens,S. moellendorffii) 的CBP60在功能上与Arabidopsis CBP60b相同,这表明CBP60b分类代表了祖先的TFs.
- 番茄和黄瓜CBP60b类基因的异质表达拯救了Arabidopsis cbp60b缺陷,并激活了这些物种的防御基因 (SID2,EDS1),证实了进化保护.
结论:
- 转录因子的CBP60b分类在整个植物进化过程中被功能性地保存.
- 这些保存的TFs在调解植物免疫力方面发挥着积极作用.
- 该CBP60b类可能代表了整个CBP60家族的原型TF.
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