质谱成像技术:非环境和环境离子化方法
Shundi Hu1,2, Ahsan Habib1,3, Wei Xiong1,2
1The Research Institute of Advanced Technologies, Ningbo University, Ningbo, Zhejiang, China.
Critical reviews in analytical chemistry
|June 18, 2024
概括
质谱成像 (MSI) 提供具有高空间分辨率的实时分子表面分析. 本综述探讨了各种MSI技术,它们的应用以及适用于生物组织诊断的适用性.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 质谱成像 (MSI) 是一种强大的技术,用于分析样品表面的分子分布.
- 它提供高空间分辨率和对多个化合物的同时信息.
- 在过去的几十年里,MSI在各种科学领域获得了显著的关注.
研究的目的:
- 审查采样协议,工作原则和MSI技术的应用.
- 为了比较非环境和环境电离MSI方法.
- 评估MSI适用于生物样本分析的适用性,特别是在组织诊断中.
主要方法:
- 非环境MSI技术的描述:二次电离质谱法 (SIMS) 和矩阵辅助激光溶解/电离法 (MALDI).
- 环境MSI技术的描述:溶解电喷射电离 (DESI),激光剥离电喷射电离 (LAESI),探测器电喷射电离 (PESI),溶解大气压光电离 (DAPPI) 和五秒激光溶解电离 (FLDI).
- 对环境和非环境MSI的优缺点进行比较分析.
主要成果:
- MSI允许实时从样本表面获取分子信息.
- 环境和非环境MSI技术都为分子成像提供了明显的优势.
- 技术的选择取决于具体的应用,特别是对于生物样本.
结论:
- MSI是一种革命性的技术,在科学研究中具有广泛的应用.
- 环境电离技术为分析包括生物组织在内的多样样样本提供了增强的多功能性.
- 未来的前景包括应对挑战,进一步推进MSI能力在诸如组织诊断等领域.
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