增加光Oligo标记抗体的信号强度,以实现组合复杂化
Madeline E McCarthy1, Xiaoming Lu1, Oluwaferanmi Ogunleye2
1Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, South Carolina 29634-0002, United States.
Bioconjugate chemistry
|June 18, 2024
概括
内部光体修饰显著提高了用于光谱流细胞计的标抗体的信号强度,从而为生物和诊断应用提供了增强的复杂化.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 分析化学 分析化学
背景情况:
- 全频谱流细胞计提高了多重复合能力.
- 目前使用基于oligo的抗体标记和光谱成像 (MuSIC) 的方法很有前途,但信号强度低.
研究的目的:
- 为了提高形标记抗体的信号强度,用于光谱流细胞计.
- 为了研究内部与外部光体标签对抗体信号的影响.
主要方法:
- 使用外部和内部光体修饰,将传统标记的抗体与oligo标记的抗体进行比较.
- 利用光谱光度,时间分辨率光和光相关谱法来评估信号强度和亮度.
- 在用人类外周血液单核细胞进行的光谱流细胞测试实验中验证了改进的内部标记方法.
主要成果:
- 与外部标签相比,内部光体修改导致信号强度增加了6倍.
- 在外部配置中,由于静态火造成了约3倍的亮度差异.
- 内部的MuSIC探针保持了信号强度,并准确量化了CD8+淋巴细胞,使多个光体的多重复合成为可能.
结论:
- 内部光体修饰是一种可行的策略,可以在光谱流细胞计中增强Oligo标记抗体的信号强度.
- 这种改进的抗体标记方法广泛适用于使用光谱检测的各种生物和诊断应用.
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