在双成分脂质混合物中通过自发曲率变异的软化
Amirali Hossein1, Andrew H Beaven1,2, Kayla Sapp1
1Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland 20892, United States.
The journal of physical chemistry. B
|June 18, 2024
概括
脂质双层的刚性不仅仅是厚度. 脂质头组相互作用和聚类显著影响膜力学,减少曲模量并影响生物膜行为.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 计算化学计算化学
背景情况:
- 脂质双层曲模块传统上与厚度有关.
- 膜力学的分子级决定因素是复杂的.
- 了解脂质相互作用对于膜功能至关重要.
研究的目的:
- 调查脂质双层硬度超越厚度的分子决定因素.
- 在膜力学中分析脂质头组相互作用和聚类的作用.
- 评估不同力场对模拟膜刚性的影响.
主要方法:
- 使用马蒂尼2,马蒂尼3和CHARMM C36力场进行分子动力学模拟.
- 分析脂质头组动态,短暂聚类和曲率灵敏度.
- 显而易见的曲模量和膜波动的量化.
主要成果:
- 脂质头组相互作用和短暂集群被确定为硬度的关键调节器.
- 脂质集群强烈合到负曲率,诱导波浪模式.
- 与全原子模型相比,粗粒马蒂尼模型显示出更大的聚类和膜软化.
- 模拟显示了不同力场中这种效应的不同程度.
结论:
- 膜度受到脂质曲率灵敏度和动态聚类的变异的影响.
- 脂质聚类显著降低了脂质双层的明显曲模量.
- 强力场的选择对模拟膜机械特性和脂质行为产生了重大影响.
更多相关视频
09:29Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
Published on: January 19, 2020
8.4K
07:54Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
Published on: October 15, 2015
8.1K
相关概念视频
01:23Membrane Fluidity
152.0K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
152.0K
01:15Mechanisms of Membrane-bending
2.7K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.7K